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ASNA1 Is Essential for Cardiac Development and Function by Regulating Tail-Anchored Protein Stability and Vesicular Transport in Cardiomyocytes

ABSTRACT: Proteomic analysis was performed on ventricular tissues isolated from embryonic day 14.5 (E14.5) cardiomyocyte-specific Asna1 conditional knockout mice and littermate controls. Heart lysates were digested with Trypsin/Lys-C and analyzed by LC-MS/MS using an EASY-nanoLC system coupled to an Orbitrap Fusion Lumos mass spectrometer. Label-free quantification (LFQ) was used to identify differentially expressed proteins associated with tail-anchored protein targeting, vesicular trafficking, and ER-Golgi transport pathways.

INSTRUMENT(S): Orbitrap Fusion Lumos Tribrid Mass Spectrometer

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: JU CHEN

PROVIDER: MSV000100049 | MassIVE | Fri Nov 28 20:47:00 GMT 2025

SECONDARY ACCESSION(S): PXD071381

REPOSITORIES: MassIVE

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