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Multi-omics Analysis Reveals New Insights into Yak Lung Fibrosis Mechanisms Under High-Altitude Adaptation

ABSTRACT:

The lung of yak is an important adaptive organ in the cold and low oxygen environment. Hypoxia can induce the pathological manifestations of pulmonary fibrosis in yak, but there was no study on hypoxia induced pulmonary fibrosis in yak. In this study full target metabolomics were used to conduct studies on normal and fibrotic lung tissues of yaks. The results showed that Palmitoyl-L-carnitine, Decanoyl-L-carnitine and O-Acetylcarnitine were significantly highly expressed in fibrotic lung, Racemethionine and L-Methionine S-oxide were significantly lower expressed in fibrotic lungs. This study revealed the molecular mechanism of anti-fibrosis of yak lung, and these findings provided a scientific basis for formulating effective prevention and control strategies, contributing to safeguarding yak health and the sustainable development of plateau animal husbandry, and providing strong support for animal health management and disease prevention and control in plateau areas.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS11648 | MetaboLights | 2025-07-07

REPOSITORIES: MetaboLights

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			Action	DRS
	neg-Lung1.raw	Raw
	neg-Lung2.raw	Raw
	neg-Lung3.raw	Raw
	neg-PF1.raw	Raw
	neg-PF2.raw	Raw

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Multi-omics Analysis Reveals New Insights into Yak Lung Fibrosis Mechanisms Under High-Altitude Adaptation

Project description:The lung of yak is an important adaptive organ in the cold and low oxygen environment. Hypoxia can induce the pathological manifestations of pulmonary fibrosis in yak, but there was no study on hypoxia induced pulmonary fibrosis in yak. In this study, lable-free proteomics was used to conduct studies on normal and fibrotic lung tissues of yaks. The results showed that RASSF4, EDC4, CTSC and FECH were significantly up-regulated differential proteins, while CYP27A1, FKBP9, RAD23A and PLSCR2 were significantly down-regulated differential proteins. These findings provided a scientific basis for formulating effective prevention and control strategies, contributing to safeguarding yak health and the sustainable development of plateau animal husbandry, and providing strong support for animal health management and disease prevention and control in plateau areas.

2024-11-09 | PXD058063 |

Exploring the mechanisms of high-altitude adaptation in yak based on single-cell transcriptome atlas.

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Project description:Purpose：Yak long-term colonization and widespread distribution across the plateau can be serve as an ideal natural animal model to provide insights into the adaptive evolution of other plateau species, including humans. Methods:To exploring the molecular mechanisms of lung tissue in yak to response to hypoxia, the mRNA, lncRNA and miRNA of lung tissue from cattle and three different altitude yaks were sequenced. Results:A total of 21764 mRNAs, 14168 lncRNAs and 1209miRNAs (305 known and 904 novel miRNAs)were identifed.Compared yak with cattle, 4975 mRNAs, 3326 lncRNAs and 75 miRNAs were differentially expressed. 756 mRNAs, 346 lncRNAs and 83 miRNAs were found to be differentially expressed amongthree different altitude yaks(fold change≥2 and P-value<0.05). Conclusions:The differentially expressed genes were functionally enriched in long-chain fatty acid metabolic process and protein processing between yak and cattle, while the immune response and cell cycle were enriched among three different altitude yaks. Furthermore, the competing endogenous RNAs (ceRNAs) networks were identified to illustrate their roles.

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Molecular mechanisms in lung tissue of yak provide insights into high-altitude adaptation by transcriptome-wide analysis [RNA-seq]

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