Response Mechanisms of Hepatopancreatic Metabolism and Gut Microbiota in Chinese Mitten Crab (Eriocheir sinensis) Under Chronic High-pH Stress

ABSTRACT:

This study subjected Eriocheir sinensis to chronic high-pH stress (control: pH=8; experimental groups: pH=9 and pH=10) and integrated hepatopancreas transcriptomic/metabolomic analyses with intestinal microbiota 16S rRNA sequencing to explore its adaptive mechanisms. The results revealed distinct adaptive strategies across stress phases: under short-term (15 days) high-pH stress, E. sinensis maintained somatic homeostasis mainly via rapid regulation of carbohydrate, lipid, protein metabolism, and energy allocation. In contrast, long-term (30 days) stress drove a strategic shift toward accelerated lipid catabolism, enhanced energy metabolism, and activated immune-related pathways. Additionally, high-pH stress significantly altered the intestinal microbiota community structure, marked by increased abundances of Proteobacteria and Pseudomonas, indicating a potential risk of microbial dysbiosis. Collectively, these findings elucidate the physiological adaptation mechanisms of E. sinensis to high-pH environments and lay a theoretical foundation for improving the sustainability of its aquaculture under extreme pH conditions.

INSTRUMENT(S): Liquid Chromatography MS -, Liquid Chromatography MS - alternating - hilic

PROVIDER: MTBLS13708 | MetaboLights | 2026-07-04

REPOSITORIES: MetaboLights