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ABSTRACT: Effect of chronic pH stress on Eriocheir sinensis juveniles

PROVIDER: PRJNA554226 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR9673750.fastq.gz	Fastqsanger.gz
	SRR9673751.fastq.gz	Fastqsanger.gz
	SRR9673752.fastq.gz	Fastqsanger.gz
	SRR9673753.fastq.gz	Fastqsanger.gz
	SRR9673754.fastq.gz	Fastqsanger.gz

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Project description:<p>This study subjected Eriocheir sinensis to chronic high-pH stress (control: pH=8; experimental groups: pH=9 and pH=10) and integrated hepatopancreas transcriptomic/metabolomic analyses with intestinal microbiota 16S rRNA sequencing to explore its adaptive mechanisms. The results revealed distinct adaptive strategies across stress phases: under short-term (15 days) high-pH stress, E. sinensis maintained somatic homeostasis mainly via rapid regulation of carbohydrate, lipid, protein metabolism, and energy allocation. In contrast, long-term (30 days) stress drove a strategic shift toward accelerated lipid catabolism, enhanced energy metabolism, and activated immune-related pathways. Additionally, high-pH stress significantly altered the intestinal microbiota community structure, marked by increased abundances of Proteobacteria and Pseudomonas, indicating a potential risk of microbial dysbiosis. Collectively, these findings elucidate the physiological adaptation mechanisms of E. sinensis to high-pH environments and lay a theoretical foundation for improving the sustainability of its aquaculture under extreme pH conditions.</p>

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