HUMAN Ring Trial - Standards detection - Reverse phase

ABSTRACT:

To better understand the effect of different LC-MS setups on metabolite detection, five laboratories analyzed 1298 standard compounds obtained from the MetaSci metabolite standard library, classified as 'Human Endosome' (HE), 'Food Exposome' (FE), 'Chemical Exposome' (CE), 'Microbiota Exposome' (ME), 'Plant Exposome' (PE), and 'Eukaryotic Exposome' (EE), using a common reference reverse-phase method in both positive and negative ionization modes and different in-house methods. NAPS were used for alignment and retention time indexing.

In our laboratory, we ran the reference reverse-phase method, an in-house reverse-phase method, and an in-house HILIC method, all three with positive and negative ionization, using an LC-MS with a Q-ToF analyzer. In this study, we present data from the reference reverse-phase method and the in-house reverse-phase method.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS14343 | MetaboLights | 2026-04-24

REPOSITORIES: MetaboLights