Project description:UHPLC-HRMS/MS data of murine colon tissue in positive and negative mode. Metabolomics. Reverse phase chromatography using F5 column. Iterative DDA data collected using AcquireX procedure on Thermo MS.
Project description:<p>The data are dissolved organic compounds from three stations sampled in the western Atlantic Ocean in 2013. Seawater samples spanning from the surface ocean to the seafloor were collected, filtered, acidified, and extracted using solid phase extraction with Bond Elut PPL cartridges. The extracts were separated using reversed phase chromatography and analyzed with ultrahigh resolution mass spectrometry in negative ion mode. The resulting data are features, or a unique combination of mass-to -charge values and retention time. </p>
Project description:analysis of drugs from the dark web. analyzed by positive and negative mode electrospray ionization. UPHLC-MS. reverse-phase chromatography (C18).
Project description:<p>To better understand the effect of different LC-MS setups on metabolite detection, five laboratories analyzed 1298 standard compounds obtained from the MetaSci metabolite standard library, classified as 'Human Endosome' (HE), 'Food Exposome' (FE), 'Chemical Exposome' (CE), 'Microbiota Exposome' (ME), 'Plant Exposome' (PE), and 'Eukaryotic Exposome' (EE), using a common reference reverse-phase method in both positive and negative ionization modes and different in-house methods. NAPS were used for alignment and retention time indexing.</p><p>In our laboratory, we ran the reference reverse-phase method, an in-house reverse-phase method, and an in-house HILIC method, all three with positive and negative ionization, using an LC-MS with a Q-ToF analyzer. In this study, we present data from the reference reverse-phase method and the in-house reverse-phase method.</p>
Project description:A glycoproteomic analysis of trypsin digested N-glycopeptides. The sample was isolated from an exosome preparation of human urine. Glycopeptides were enriched using lectin chromatography, fractionated by high-pH reverse phase chromatography and analyzed offline on an Orbitrap Fusion Lumos.
Project description:Interventions: Gold Standard:pathological diagnosis ;Index test:Volatile organic compounds detected by gas chromatography-mass spectrometer and gas chromatography-ion migration spectrometry
Primary outcome(s): Volatile organic compounds;sensitivity;specificity
Study Design: Diagnostic test for accuracy
Project description:Advancing Negative Ion Mode Proteomics. The main objective of the project is the exploration of the unconvetional negative ion mode for proteomics studies. In this work, we thoroughly studied the best chromatographic conditions for negative ion mode proteomics before testing different enzymatic digestion. The final goal is to establish the best working conditions in the negative polarity for negative ion mode. The method also refrains from any fragmentation events, which are unpredictable in negative ion mode.
Project description:Here we report a simple and versatile approach for domain mapping of complex mixtures of glycosaminoglycans (GAGs), GAGDoMa. The approach is based on orthogonal enzymatic depolymerization of the GAGs, generating internal oligosaccharide, non-reducing end, and linkage region GAG domains, nanoflow reversed-phase dibutylamine ion-pairing chromatography and negative mode higher-energy collision dissociation (HCD) MS/MS. GAGDoMa provides a detailed insight into the GAGome, and will be an important tool for the understanding of GAGs in cellular physiology and pathology.
Project description:Human islets were treated with IL-1b and IFN-g for 24h, digested with trypsin, multiplexed with TMT-10, fractionated by high pH reverse-phase chromatography and analyzed by tandem LC-MS/MS.