Dataset Information

Glycosaminoglycan Domain Mapping of cellular chondroitin/dermatan sulfates

ABSTRACT: Here we report a simple and versatile approach for domain mapping of complex mixtures of glycosaminoglycans (GAGs), GAGDoMa. The approach is based on orthogonal enzymatic depolymerization of the GAGs, generating internal oligosaccharide, non-reducing end, and linkage region GAG domains, nanoflow reversed-phase dibutylamine ion-pairing chromatography and negative mode higher-energy collision dissociation (HCD) MS/MS. GAGDoMa provides a detailed insight into the GAGome, and will be an important tool for the understanding of GAGs in cellular physiology and pathology.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Egor Vorontsov

LAB HEAD: Jonas Nilsson

PROVIDER: PXD014504 | Pride | 2020-02-18

REPOSITORIES: Pride

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Glycosaminoglycan Domain Mapping of Cellular Chondroitin/Dermatan Sulfates.

Persson Andrea A Vorontsov Egor E Larson Göran G Nilsson Jonas J

Scientific reports 20200226 1

Glycosaminoglycans (GAGs) are polysaccharides produced by most mammalian cells and involved in a variety of biological processes. However, due to the size and complexity of GAGs, detailed knowledge about the structure and expression of GAGs by cells, the glycosaminoglycome, is lacking. Here we report a straightforward and versatile approach for structural domain mapping of complex mixtures of GAGs, GAGDoMa. The approach is based on orthogonal enzymatic depolymerization of the GAGs to generate in ...[more]

PMID: 32103093

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Dataset Information

Glycosaminoglycan Domain Mapping of cellular chondroitin/dermatan sulfates

Publications

Glycosaminoglycan Domain Mapping of Cellular Chondroitin/Dermatan Sulfates.

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