Glycosaminoglycan Domain Mapping of cellular chondroitin/dermatan sulfates

ABSTRACT: Here we report a simple and versatile approach for domain mapping of complex mixtures of glycosaminoglycans (GAGs), GAGDoMa. The approach is based on orthogonal enzymatic depolymerization of the GAGs, generating internal oligosaccharide, non-reducing end, and linkage region GAG domains, nanoflow reversed-phase dibutylamine ion-pairing chromatography and negative mode higher-energy collision dissociation (HCD) MS/MS. GAGDoMa provides a detailed insight into the GAGome, and will be an important tool for the understanding of GAGs in cellular physiology and pathology.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Egor Vorontsov  

LAB HEAD: Jonas Nilsson

PROVIDER: PXD014504 | Pride | 2020-02-18

REPOSITORIES: Pride