Omics-guided global rewiring of yeast metabolism enables high-level production of β-lactoglobulin

ABSTRACT: This project contains untargeted metabolomic data generated to investigate the metabolic responses of Komagataella phaffii during methanol-free recombinant beta-lactoglobulin production. The study included the bovine beta-lactoglobulin-producing strain B08 and the feline beta-lactoglobulin-producing strain F01. Samples were collected under glucose and ethanol conditions at 36 h and 84 h of fermentation, with biological replicates. Untargeted metabolomic profiling was performed by LC-MS/MS in both positive and negative ion modes. The dataset was used to compare host metabolic changes associated with carbon source, fermentation stage, and beta-lactoglobulin species origin, with particular focus on central carbon metabolism, energy metabolism, amino acid metabolism, redox homeostasis, and protein synthesis and secretion. These data support multi-omics integration and metabolic engineering target selection for improving recombinant beta-lactoglobulin production in K. phaffii.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse-phase, Liquid Chromatography MS - positive - reverse-phase

PROVIDER: MTBLS14680 | MetaboLights | 2026-06-05

REPOSITORIES: MetaboLights