Dataset Information

A red wine intervention does not modify plasma trimethylamine N-oxide but is associated with broad shifts in the plasma metabolome and gut microbiota composition

ABSTRACT:

BACKGROUND:

Gut microbiota profiles are closely related to cardiovascular diseases through mechanisms that include the reported deleterious effects of metabolites, such as trimethylamine N-oxide (TMAO), which have been studied as diagnostic and therapeutic targets. Moderate red wine (RW) consumption is reportedly cardioprotective, possibly by affecting the gut microbiota.

OBJECTIVES:

To investigate the effects of RW consumption on the gut microbiota, plasma TMAO, and the plasma metabolome in men with documented coronary artery disease (CAD) using a multiomics assessment in a crossover trial.

METHODS:

We conducted a randomized, crossover, controlled trial involving 42 men (average age, 60 y) with documented CAD comparing 3-wk RW consumption (250 mL/d, 5 d/wk) with an equal period of alcohol abstention, both preceded by a 2-wk washout period. The gut microbiota was analyzed via 16S rRNA high-throughput sequencing. Plasma TMAO was evaluated by LC-MS/MS. The plasma metabolome of 20 randomly selected participants was evaluated by ultra-high-performance LC-MS/MS. The effect of RW consumption was assessed by individual comparisons using paired tests during the abstention and RW periods.

RESULTS:

Plasma TMAO did not differ between RW intervention and alcohol abstention, and TMAO concentrations showed low intraindividual concordance over time, with an intraclass correlation coefficient of 0.049 during the control period. After RW consumption, there was significant remodeling of the gut microbiota, with a difference in β diversity and predominance of Parasutterella, Ruminococcaceae, several Bacteroides species, and Prevotella. Plasma metabolomic analysis revealed significant changes in metabolites after RW consumption, consistent with improved redox homeostasis.

CONCLUSIONS:

Modulation of the gut microbiota may contribute to the putative cardiovascular benefits of moderate RW consumption. The low intraindividual concordance of TMAO presents challenges regarding its role as a cardiovascular risk biomarker at the individual level. This study was registered at clinical trials.gov as NCT03232099.

INSTRUMENT(S): Q Exactive

SUBMITTER: Elisa Haas Aline M A Martins

PROVIDER: MTBLS310 | MetaboLights | 2024-05-09

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS310	Other
	FILES	Other
	a_MTBLS310_Method1_POS_1_metabolite_profiling_mass_spectrometry.txt	Txt
	a_MTBLS310_Method2_POS_2_metabolite_profiling_mass_spectrometry.txt	Txt
	a_MTBLS310_Method3_NEG_1_metabolite_profiling_mass_spectrometry.txt	Txt

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Publications

A red wine intervention does not modify plasma trimethylamine N-oxide but is associated with broad shifts in the plasma metabolome and gut microbiota composition.

Haas Elisa A EA Saad Mario J A MJA Santos Andrey A Vitulo Nicola N Lemos Wilson J F WJF Martins Aline M A AMA Picossi Carolina R C CRC Favarato Desidério D Gaspar Renato S RS Magro Daniéla O DO Libby Peter P Laurindo Francisco R M FRM Da Luz Protasio L PL

The American journal of clinical nutrition 20221201 6

<h4>Background</h4>Gut microbiota profiles are closely related to cardiovascular diseases through mechanisms that include the reported deleterious effects of metabolites, such as trimethylamine N-oxide (TMAO), which have been studied as diagnostic and therapeutic targets. Moderate red wine (RW) consumption is reportedly cardioprotective, possibly by affecting the gut microbiota.<h4>Objectives</h4>To investigate the effects of RW consumption on the gut microbiota, plasma TMAO, and the plasma meta ...[more]

PMID: 36205549

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