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Fructosamine-3-kinase (FN3K) KO in HepG2 liver cancer cells

ABSTRACT: Fructosamine-3-kinases (FN3Ks) are a family of metabolic kinases which are evolutionarily related to eukaryotic protein kinases. Aberrant regulation of these kinases by altered redox homeostasis is a major contributing factor in aging and disease. However, the mechanisms of regulation and cellular functions of these kinases are not known. Bioinformatic analyses of cancer cell lines identified significant overexpression of FN3K in liver and eye cancer cells. To assess the functional significance of this increased expression, a CRISPR knockout of FN3K (FN3K-KO) was generated in the HepG2 liver cancer cell line. The metabolome was compared between FN3K-KO and WT HepG2 cells using untargeted 1H NMR metabolomics. This revealed significant differences in several metabolites that suggest a role for FN3K in regulating redox and energy balance in HepG2 cells.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Hep G2 Cells

SUBMITTER: Maxwell Colonna

PROVIDER: ST001375 | MetabolomicsWorkbench | Fri May 08 00:00:00 BST 2020

REPOSITORIES: MetabolomicsWorkbench

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