Timecourse of NIH-3T3 cells treated with apoptotic inducers
Ontology highlight
ABSTRACT: Time-dependent changes of phosphatidylinositol profiles in mouse NIH-3T3 fibroblasts treated with mechanistically diverse apoptotic inducers.
Project description:Expression profiling of HepG2 human liver carcinoma cells and NIH 3T3 mouse fibroblasts after arsite treatment for 24h. RNA-seq data comprise 4 groups: NIH 3T3 mouse fibroblasts control and arsite treatment, and HepG2 human liver carcinoma cells control and arsenite treatment. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:Analysis of Immediate Early Response 2 (Ier2)-inducible NIH 3T3 cells after Ier2 induction with RheoSwitch ligand RSL-1. Results provide insight into the function of Ier2 in NIH 3T3 mouse embryonal fibroblasts. Immediate early genes, including Ier2, are rapidly induced in quiescent cells by proliferation and migration-inducing stimuli. Microarray gene expression profiling was performed to identify differentially expressed genes following overexpression of Ier2 in NIH 3T3-Ier2 inducible cells after 24 hour induction of Ier2.
Project description:Aldoa knockdown (using a pool of 4 siRNAs) vs. non-targeting control knockdown (using a pool of 4 siRNAs) in murine NIH/3T3 cells (fibroblasts). Cells were harvested 48 hours after transfection.
Project description:To better understand the extent to which induced pluripotent stem cells (iPSCs) faithfully recapitulate the characteristics of embryonic stem cells (ESCs) under (undiff)erentiated condition, KSR condition and FBS condition and how both compare to somatic tissues under these conditions, we employed whole-genome transcriptome analysis on all twenty one hESC lines available on the pre-2008 NIH Human Pluripotent Stem Cell Registry, eight human iPSCs derived at NIH by retroviral transduction of human fibroblasts and twenty human somatic tissues. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used and are available at: http://stemcelldb.nih.gov.
Project description:Transcriptomics of NIH-3T3 cells treated with typical or atypical RARa modulators to evaluate genetic changes induced by chemical modification of RARa signalling
Project description:The interaction of macrophages with apoptotic cells is required for efficient resolution of inflammation. While apoptotic cell removal prevents inflammation due to secondary necrosis, it also alters the macrophage phenotype to hinder further inflammatory reactions. The interaction between apoptotic cells and macrophages is often studied by chemical or biological induction of apoptosis, which may introduce artifacts by affecting the macrophages as well and/or triggering unrelated signaling pathways. Here, we set up a pure cell death system in which NIH 3T3 cells expressing dimerizable Caspase-8 were co-cultured with peritoneal macrophages in a transwell system. Phenotype changes in macrophages induced by apoptotic cells were evaluated by RNA sequencing, which revealed an unexpectedly dominant impact on macrophage proliferation. This was confirmed in functional assays with primary peritoneal macrophages and IC-21 macrophages. Moreover, inhibition of apoptosis during Zymosan-induced peritonitis in mice decreased mRNA levels of cell cycle mediators in peritoneal macrophages. Proliferation of macrophages in response to apoptotic cells may be important to increase macrophage numbers to allow efficient clearance and resolution of inflammation.
Project description:This SuperSeries is composed of the following subset Series: GSE32923: The NIH Human Pluripotent Stem Cell Database (Agilent, mRNA) GSE33789: The NIH Human Pluripotent Stem Cell Database (Affymetrix, mRNA) GSE34199: The NIH Human Pluripotent Stem Cell Database (Agilent, miRNA) GSE34869: The NIH Human Pluripotent Stem Cell Database (Illumina, methylation) GSE35157: The NIH Human Pluripotent Stem Cell Database (Illumina, snp) GSE35735: The NIH Human Pluripotent Stem Cell Database (Agilent, cgh) Refer to individual Series