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P53 K316P mutation results in increased liver triglyceride levels and increased rates of de novo lipogenesis.

ABSTRACT: Our lab generated the p53 K316P mouse, which mimicks a common amino acid change found in bats. The K316P mutation, found in the nuclear localization signal of p53, results in increased cytoplasmic localization of p53. We found that K316P mutant mice develop several metabolic phenotypes, including increased body fat percentage, and increased liver lipid levels. In order to determine the mechanism through which K316P mutation increases liver lipid levels, we performed metabolomic analysis of mouse livers from WT and K316P mutant mice. Mouse livers were isolated from four wild type (WT) and four p53 K316P (M) mice for lipidomic analysis. Samples were isolated and flash frozen in liquid nitrogen. Lipids were then extracted from each liver sample and analyzed using mass spectrometry.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Liver

DISEASE(S): Obesity

SUBMITTER: Jack Sanford

PROVIDER: ST002396 | MetabolomicsWorkbench | Wed Dec 07 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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