Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: PRJNA432936
TISSUE(S): 293ft Cells
SUBMITTER: Li,Yang
REPOSITORIES: NODE
Li Xiaosa X Wang Ying Y Liu Yajing Y Yang Bei B Wang Xiao X Wei Jia J Lu Zongyang Z Zhang Yuxi Y Wu Jing J Huang Xingxu X Yang Li L Chen Jia J
Nature biotechnology 20180319 4
The targeting range of CRISPR-Cas9 base editors (BEs) is limited by their G/C-rich protospacer-adjacent motif (PAM) sequences. To overcome this limitation, we developed a CRISPR-Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. The base editor recognizes a T-rich PAM sequence and catalyzes C-to-T conversion in human cells, while inducing low levels of indels, non-C-to-T substitutions and off-target editing. ...[more]