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OEP_Jianxiang_1909271315

ABSTRACT: EC109 cells and TE-1 cells were cultured in Dulbecco's modified Eagle medium (DMEM) and RPMI 1640 medium, respectively, supplemented with 10% FBS, 100 U ml-1 penicillin and 100 mg/ml streptomycin. EC109 cells and TE1 cells were treated with DMSO or oridonin（30μM）, respectively, and total cellular RNA was extracted for RNA-seq. Total RNA from three duplicate wells per group were extracted by using RNA prep Pure Cell/Bacteria Kit（Qiagen) according to the manufacturer's instructions. RNA-seq cDNA library was prepared using the TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina) according to the manufacturer's instructions.Briefly, total RNA was extracted for mRNA purification and fragmentation, first strand cDNA synthesis, second strand cDNA synthesis, adenylate 3' end, ligation linker and PCR amplification library.

TISSUE(S): Escc Cell Lines

SUBMITTER: Jianxiang,Shi

PROVIDER: OEX001952 | NODE |

REPOSITORIES: NODE

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