Project description:Lotus japonicus is a model legume broadly used to study transcriptome regulation under different stress conditions and microorganism interaction. Understanding how this model plant respond gainst alkaline stress will certainly help to develop more tolerant cultivars in economically important Lotus species as well as in other legumes. In order to uncover the most important response mechanisms activated during alkaline stress, we explored by microarray analysis the transcriptome regulation occurring in the phenotypically contrasting ecotypes MG-20 and Gifu B-129 of L. japonicus after 21 days of alkaline stress.

2014-03-25 | E-MTAB-2418 | biostudies-arrayexpress

Lotus japonicus phosphopeptide

Project description:Phosphopeptides were purified using Titansphere. Before MS analysis, phosphopeptides were desalted using StageTips with C18 Empore disk membranes. Phosphopeptides were analysed by Orbitrap MS.The obtained MS and MS/MS spectra data were searched against Kazusa DNA Research Institute published lotus EST database using the MASCOT program (FDR<1%).

2013-10-29 | PXD000053 | Pride

Chromosomal map of the model legume Lotus japonicus.

Project description: Not available

| S-EPMC1462223 | biostudies-other

Tissue-specific transcriptome analysis in nodules of Lotus japonicus

Project description:Legume plants can establish symbiotic nitrogen fixation (SNF) with rhizobia mostly in root nodules, where rhizobia-infected cells are accompanied with uninfected cells in a mosaic pattern. Inside the mature nodules of legume, carbon and nitrogen nutrients between host plant cells and their resident bacteria are actively exchanged. To elucidate the metabolite dynamics relevant for SNF in nodules, three cell-types from nodule tissues of a model legume, Lotus japonicus, were isolated using laser microdissesction, and transcriptome analysis was done by an oligoarray with 60-mer length representing 21,495 genes. In our cell-type-specific profiling, many genes were identified as being expressed in nodules with spatial-specific manners. Among them, genes coding for metabolic enzymes were classified according to their function, and detailed data analysis figured out that secondary metabolic pathway was highly activated in nodule cortex. In particular, a number of metabolic genes for phenyl propanoid pathway were found as highly expressed genes accompanied with those encoding putative transporters of secondary metabolites. These data suggest the involvement of novel physiological function of phenylpropanoids in SNF.

2011-12-29 | GSE34753 | GEO

Methylome of Lotus japonicus accession 'Gifu'

Project description:Whole genome DNA methylation data for the the Lotus japonicus accession 'Gifu'

2016-12-01 | GSE73069 | GEO

Genome structure of the legume, Lotus japonicus.

Project description: Not available

| S-EPMC2575887 | biostudies-literature