Proteomics

Dataset Information

98

Lotus japonicus phosphopeptide


ABSTRACT: Phosphopeptides were purified using Titansphere. Before MS analysis, phosphopeptides were desalted using StageTips with C18 Empore disk membranes. Phosphopeptides were analysed by Orbitrap MS.The obtained MS and MS/MS spectra data were searched against Kazusa DNA Research Institute published lotus EST database using the MASCOT program (FDR<1%).

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Lotus Japonicus

SUBMITTER: Yoko Ino  

PROVIDER: PXD000053 | Pride | 2013-10-29

REPOSITORIES: Pride

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Publications

Phosphoproteome analysis of Lotus japonicus seeds.

Ino Yoko Y   Ishikawa Akiyo A   Nomura Ayako A   Kajiwara Hideyuki H   Harada Kyuya K   Hirano Hisashi H  

Proteomics 20140101 1


In this study, we report the first dataset of phosphoproteins of the seeds of a model plant, Lotus japonicus. This dataset might be useful in studying the regulatory mechanisms of seed germination in legume plants. By proteomic analysis of seeds following water absorption, we identified a total of 721 phosphopeptides derived from 343 phosphoproteins in cotyledons, and 931 phosphopeptides from 473 phosphoproteins in hypocotyls. Kinase-specific prediction analyses revealed that different kinases w  ...[more]

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