Project description:Biological treatments to degrade cyanide are a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal-cyanide complexes as the sole nitrogen source. In this work, the strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. To elucidate the molecular mechanisms involved in the bioremediation process, a quantitative sRNA sequencing analysis has been carried out in P. pseudoalcaligenes CECT5344 cells grown with the jewelry residue, free cyanide and ammonium as sole nitrogen sources.
Project description:Transcriptional analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to cyanide present in wastewaters from industrial activities, such as jewelry and electroplating activities
Project description:Transcriptional analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to cyanide present in wastewaters from industrial activities, such as jewelry and electroplating activities Four-conditions experiment, including three different nitrogen sources (ammonium, sodium cyanide or a cyanide-containing wastewater). One experiment without nitrogen added to the media (nitrogen limited condition). Four biological replicates for each condition (added nitrogen source to the media) plus the experiment without nitrogen source added.
Project description:Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 to compare the nitrogen metabolism when ammonium, beta-cyanoalanine and jewelry residue (with both free and metal-complexed cyanide) are used as sole nitrogen source
Project description:Biological treatments to degrade cyanide have shown to be a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal−cyanide complexes as the sole nitrogen source. The strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the electroplating process of the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. A proteomic analysis by LC-MS/MS has been applied to elucidate the molecular mechanisms involved in this bioremediation process in P. pseudoalcaligenes CECT5344. Among others, different proteins related to cyanide and cyanate assimilation, as well as other proteins involved in transport and resistance to metals were induced by the cyanide-containing jewelry residue have been identified.
Project description:Quantitative free labeling proteomics to elucidate the role of the DapA protein in iron metabolism and the assimilation of cyanide in the bacterium Pseudomonas pseudoalcaligenes. Integration, validation and discussion of results using other non-proteomic techniques such as qRT-PCR and determination of key metals.
Project description:By screening the secretomes of polymer induced Pseudomonas pseudoalcaligenes we identify a new enzyme PpEst that can degrade the co-aliphatic-aromatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT). The discovered enzyme has predicted arylesterase activity and is induced by PBAT added to the growth medium
Project description:Quantitative proteomic analysis of P. pseudoalcaligenes CECT 5344 in the presence of arsenite (As(III)) with ammonium and cyanide as nitrogen sources
