Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cyanoalanine in Pseudomonas pseudoalcaligenes CECT5344

ABSTRACT: Quantitative proteomic analysis of Pseudomonas pseudoalcaligenes CECT5344 to compare the nitrogen metabolism when ammonium, beta-cyanoalanine and jewelry residue (with both free and metal-complexed cyanide) are used as sole nitrogen source

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Pseudomonas Pseudoalcaligenes Cect 5344

TISSUE(S): Prokaryotic Cell

SUBMITTER: Alfonso Olaya Abril

LAB HEAD: Conrado Moreno Vivián

PROVIDER: PXD026640 | Pride | 2022-02-17

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Alternative Pathway for 3-Cyanoalanine Assimilation in Pseudomonas pseudoalcaligenes CECT5344 under Noncyanotrophic Conditions.

Pérez María D MD Olaya-Abril Alfonso A Cabello Purificación P Sáez Lara P LP Roldán M Dolores MD Moreno-Vivián Conrado C Luque-Almagro Víctor M VM

Microbiology spectrum 20211103 3

3-Cyanoalanine and cyanohydrins are intermediate nitriles produced in cyanide degradation pathways in plants and bacteria. 3-Cyanoalanine is generated from cyanide by the 3-cyanoalanine synthase, an enzyme mainly characterized in cyanogenic plants. NIT4-type nitrilases use 3-cyanoalanine as a substrate, forming ammonium and aspartate. In some organisms, this enzyme also generates asparagine through an additional nitrile hydratase activity. The alkaliphilic bacterium Pseudomonas pseudoalcaligenes ...[more]

PMID: 34730416

Similar Datasets

Regulatory non-coding sRNA in response to cyanide in Pseudomonas pseudoalcaligenes CECT5344

Project description:Biological treatments to degrade cyanide are a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal-cyanide complexes as the sole nitrogen source. In this work, the strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. To elucidate the molecular mechanisms involved in the bioremediation process, a quantitative sRNA sequencing analysis has been carried out in P. pseudoalcaligenes CECT5344 cells grown with the jewelry residue, free cyanide and ammonium as sole nitrogen sources.

2019-02-13 | GSE117374 | GEO

Proteome of arsenite in P. pseudoalcaligenes CECT 5344

Project description:Quantitative proteomic analysis of P. pseudoalcaligenes CECT 5344 in the presence of arsenite (As(III)) with ammonium and cyanide as nitrogen sources

2023-05-10 | PXD033539 | Pride

Pseudomonas pseudoalcaligenes CECT5344 response to cyanide vs. ammonium or nitrogen starvation

Project description:Transcriptional analysis of the cyanotroph Pseudomonas pseudoalcaligenes CECT5344 in response to cyanide present in wastewaters from industrial activities, such as jewelry and electroplating activities Four-conditions experiment, including three different nitrogen sources (ammonium, sodium cyanide or a cyanide-containing wastewater). One experiment without nitrogen added to the media (nitrogen limited condition). Four biological replicates for each condition (added nitrogen source to the media) plus the experiment without nitrogen source added.

2016-01-27 | E-GEOD-69930 | biostudies-arrayexpress

Proteomic response of P. pseudoalcaligenes CECT 5344 to mercury in presence of cyanideProteomic response of P. pseudoalcaligenes CECT 5344 to mercury in presence of cyanide

Project description:Quantitative proteomic analysis of P. pseudoalcaligenes CECT 5344 in the presence of mercury with cyanide as nitrogen source

2023-10-24 | PXD038659 | Pride

Quantitative proteomic analysis (LC-MS/MS) of Pseudomonas pseudoalcaligenes CECT5344 in response to industrial cyanide-containing wastewaters

Project description:Biological treatments to degrade cyanide have shown to be a powerful technology for cyanide removal from industrial wastewaters. It has been previously demonstrated that the alkaliphilic bacterium Pseudomonas pseudoalcaligenes CECT5344 is able to use free cyanide and several metal−cyanide complexes as the sole nitrogen source. The strain CECT5344 has been used for detoxification of the different chemical forms of cyanide that are present in alkaline wastewaters from the electroplating process of the jewelry industry. This liquid residue also contains large concentrations of metals like iron, copper and zinc, making this wastewater even more toxic. A proteomic analysis by LC-MS/MS has been applied to elucidate the molecular mechanisms involved in this bioremediation process in P. pseudoalcaligenes CECT5344. Among others, different proteins related to cyanide and cyanate assimilation, as well as other proteins involved in transport and resistance to metals were induced by the cyanide-containing jewelry residue have been identified.

2017-03-08 | PXD005056 | Pride

Pseudomonas pseudoalcaligenes CECT5344 response to cyanide vs. ammonium or nitrogen starvation

2016-01-27 | GSE69930 | GEO

Role of the DapA protein in iron homeostasis and the metabolism of cyanide in jewelery wastewaters

Project description:Quantitative free labeling proteomics to elucidate the role of the DapA protein in iron metabolism and the assimilation of cyanide in the bacterium Pseudomonas pseudoalcaligenes. Integration, validation and discussion of results using other non-proteomic techniques such as qRT-PCR and determination of key metals.

2020-02-18 | PXD014027 | Pride

Pseudomonas pseudoalcaligenes CECT5344

Project description:Draft whole genome sequence of the cyanide-degrading bacterium Pseudomonas pseudoalcaligenes CECT5344

| PRJEB27 | ENA

Azotobacter vinelandii str. DJ transcriptomic time-course under nitrogenase derepressing conditions

Project description:Comparison of Azotobacter vinelandii str. DJ transcriptomes using either ammonium or dinitrogen as sole nitrogen source. Transcriptomes from cells in early stages of nitrogenase derepression (10 min and 30 min) and at the peak of nitrogenase derepression (4 hours) were compared against the corresponding controls from cells using ammonium as nitrogen source. Kinetics of the nitrogen fixation (nif) genes expression profiles resemble those previously published (Poza-Carrion, 2013; doi: 10.1128/jb.00942-13).

2023-10-10 | GSE244772 | GEO

Transcriptome analysis of Arabidopsis wild-type and NRT1.1-knockout mutant seedlings grown under 10 mM ammonium as sole nitrogen source

Project description:High levels of ammonium as the sole nitrogen source impairs plant growth, i.e. ammonium toxicity, the primary cause of which remains to be determined. To obtain a clue about the toxic mechanism, we compared genome-wide gene expression between the wild-type (Col) and NRT1.1-knockout mutant (chl1-5, nrt1.1) seedlings growing in media containing toxic levels of ammonium as the sole nitrogen source.

2023-10-31 | E-MTAB-13395 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data