Project description:This SuperSeries is composed of the following subset Series: GSE32241: Differentially regulated genes induced in Mycobacterium avium subspecies paratuberculosis by in vitro acid-nitrosative multi-stress GSE32242: Differentially regulated genes induced in Mycobacterium avium subspecies paratuberculosis by in vitro infection of THP-1 human macrophage cell line Refer to individual Series
Project description:Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress.
Project description:Comparing the transcriptomic responses between the Mycobacterium avium subspecies paratuberculosis (MAP) leuD mutant with the parent strain K-10 under different environmental stresses: nutrition, temperature, anaerobic conditions, high- and low- pH conditions.
Project description:Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro THP-1 (ATCC TIB-202) cell line infection, comparing bacteria grown in host medium and intracellular bacteria recovered from infected THP-1 cells.
Project description:Mycobacterium avium is a significant opportunistic intracellular pathogen in humans, pigs and birds. Human and porcine infection with M. avium is mainly due to M. avium subsp. hominissuis, whereas birds are typically infected with M. avium subsp. avium. Reasons for this distribution of subspecies between the hosts are uncertain, but they might be due to differences in exposure or to different abilities to infect the respective hosts. We wanted to assay how isolates of the two subspecies interact with a human host in a macrophage model to gain more knowledge on possible differences in virulence. This study compares clinical isolates of M. avium with regards to their ability to invade and replicate intracellularly in human monocytes/macrophages and to the gene expression triggered in these cells upon infection. Further focus in the description of protocols is limited to the parts of the study that regards the expression analysis.
Project description:The alteration in genomic expression profiles of mRNA and long non-coding RNA (lncRNA) in bovine macrophages in response to Mycobacterium avium subspecies paratuberculosis (MAP) infection was investigated by applying an RNA-Seq based approach. A computational pipeline was implemented which identified more than 300 unannotated lncRNAs in macrophages of which 38 were differentially regulated by the infection.
Project description:This is a pathogenic mutation profile of colorectal patients specifically in 5 genes, i.e. APC, TP53, PIK3CA, KRAS, and MLH1. Single nucleotide variants identified were synchronized with patients’ characteristics.
Project description:Comparative expression profiles of 3 defined groups of Mycobacterium avium subspecies paratuberculosis (MAP) infected terminal ileum from sheep. Defined by histopathlogy and IS900 real-time PCR as multibacillary, paucibacillary and asymptomatic sheep. Six sheep per group provided biological and technical replicates on the RIGUA custom array (Watkins et al., 2008).
Project description:Comparing the transcriptomic responses between the Mycobacterium avium subspecies paratuberculosis (MAP) leuD mutant with the parent strain K-10 under different environmental stresses: nutrition, temperature, anaerobic conditions, high- and low- pH conditions. MAP wild type K-10 vs. leuD mutant, 6 different treatments. Two biological replicates with two technical replicates.
