Project description:Purpose: Global survey of the transcriptome and comparison to the proteome in autophagy-deficient strains of D. discoideum. Results: Our RNAseq analysis of ATG9‾, ATG16‾ and ATG9‾/16‾ cells revealed major transcriptional changes in a large number of genes in comparison to AX2. Conclusions: Our study provides the first combined transcriptome and proteome analysis of ATG9‾, ATG16‾ and ATG9‾/16‾ cells and adds to our knowledge of functions of these proteins in Dictyostelium discoideum.
Project description:Using RNAseq to identify differentially expressed transcripts between CBFB wild type (WT) and knockout (KO) or between RUNX1 wild type (WT) and knockout (KO) MCF10A cells.
Project description:Knockout mutants of polyketide synthase genes were generated (pks1, pks2, pks3, pks14, pks18, pks37) and compared to wild-type AX2 strain of Dictyostelium discoideum using Agilent custom microarrays. The Dictyostelium cells were harvested at eight developmental stages (Amoeboid, Intiation of starvation, loose aggregate, Streaming, Mound, Slug, early culminant, Fruiting body) and subjected to microarray based expression profiling.
Project description:Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum: comparing wild type and forG- (formin-) mutant cells dedifferentiating in growth medium
Project description:Purpose: We aim to reveal maize transcriptomic changes in wild-type and Gβ knockout lines. Methods: RNA-seq was used to reveal transcriptome of maize biological replicates of wild-type and Gβ knockout lines. Results: Differnentically expressed transcritps were identified by the comparison of biological replicates of wild-type and Gβ knockout lines. Conclusions: We identified differentially expressed genes in Gβ knockout lines.
Project description:We describe the gene expression pattern of a Dictyostelium discoideum mutant depleted in the C-module-binding factor (CbfA) and autonomous gene-regulatory activities of CbfA carboxy-terminal domains (CbfA-CTDs) Comparison of gene expression in growing cells of a CbfA mutant compared to wild-type
Project description:In this experiment, we investigated how CbrA contributed to the expression of P. aeruginosa virulence factors in vivo using microarrays. Two independent microarray analyses were performed to identify the global gene expression of the cbrA mutant in comparison to PA14 wild type strain during D. discoideum infection.
Project description:Purpose: We aim to reveal maize transcriptomic changes in wild-type and lncRNA GARR2 knockout lines. Methods: RNA-seq was used to reveal transcriptome of maize biological replicates of wild-type and lncRNA GARR2 knockout lines. Results: Differnentically expressed transcritps were identified by the comparison of biological replicates of wild-type and lncRNA GARR2 knockout lines. Conclusions: We identified differentially expressed genes in lncRNA GARR2 knockout lines.
Project description:Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum: comparing wild type with bzpS-, mybD-, nfyA-, DDB_G0281091-, DDB_G0269374- and DDB_G0272386- mutant cells dedifferentiating in growth medium
