Project description:Multi-functional capture reagents reported here enable robust identification of metabolically-tagged myristoylated proteomes with unprecedented confidence resulting from the combination of chemical probe-based enrichment, and release and direct detection of lipid-modified peptides by MS. Whilst capture reagents containing enzymatically cleavable linkers have been previously reported they typically require an extra proteolytic step and their capacity to enable detection of lipidated peptides has not been demonstrated. Herein, we report the largest database (85 counts) of experimentally validated human proteins that are myristoylated at an endogenous level in living cells. Furthermore, we demonstrate the first profile of myristoylation in a living multicellular organism and the confident identification of over 50 novel targets. Importantly, this is also the first example of analysis of any protein lipidation event during development. Our methodology is novel in analytical/chemical approach, and provides quantitative and dynamic information. This submission concerns myristoylated proteomes of zebrafish origin.

2015-03-30 | PXD001876 | Pride

Danio rerio

Project description:Spatiotemporal Analysis of Transcriptome in the paraxial mesoderm of zebrafish embryos

| PRJNA116769 | ENA

Danio rerio

Project description:Identification of mesendoderm precursor- and neurectoderm precursor-enriched genes in Zebrafish embryos

| PRJNA101849 | ENA

Danio rerio

Project description:RNA-seq for developing pectoral fin in zebrafish

| PRJDB9741 | ENA

Danio rerio

Project description:Identification of differentially-expressed genes between FoxH1 morpholino and control in zebrafish embryos.