Project description:Single cell technologies have paved the way for the characterization of colorectal cancer.We performed scRNA-seq on human colorectal tumors and their matched adjacent-normal tissues and liver metastasis tissue. The heterogneity of the tumor immune microenvironment were analyzed.
Project description:Single cell RNA-sequencing has been applied to core and border regions of 9 colorectal tumors as well as to matched adjacent non-malignant colon tissue for the purpose of generating a cellular map of colorectal tumors and their tumor microenvironment.
Project description:We performed gene expression profiling of 26 colorectal tumors and matched histologically normal adjacent colonic tissue samples using the Illumina Ref-8 whole-genome expression BeadChip. We performed an integrated analysis of promoter DNA methylation and gene expression data to investigate the effects of DNA hypermethylation on gene expression. Total RNA was isolated from 26 pairs of fresh frozen colorectal tumor and matched adjacent non-tumor tissue samples. Their gene expression profiles were obtained using the Illumina Ref-8 whole-genome expression BeadChip (HumanRef-8 v3.0, 24,526 transcripts).
Project description:lncRNAs contributes to the development of colorectal cancer (CRC). Analysis of tumor tissues and adjacent non-tumor tissues from 6 colorectal cancer patients was conducted. Results indicate insight into molecular signature of the tumorigenesis of CRC.
Project description:Total RNA from 6 pairs colorectal cancer tissues and its matched adjacent non-cancerous tissues was extracted and sequenced by high throughput sequencing. Then, differentially expressed circRNAs, long noncoding RNAs, and mRNAs were showed in colorectal cancer.