Saccharomyces cerevisiae
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ABSTRACT:
PROVIDER: PRJNA227050 | ENA |
REPOSITORIES: ENA
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Project description:A TATA binding protein regulatory network
| PRJNA97865 | ENA
Project description:We constructed a non-flocculating strain PLY01 from industrial flocculating strain SPSC01. In the presence of 5.0 g/L acetic acid stress, SPSC01 showed better growth and fermentation performance compared to PLY01. For probing mechanism of improved acetic acid tolerance induced by flocculation, we performed proteomic analysis to mining key proteins and regulation network.
2021-09-06 | PXD028214 | iProX
Project description:High concenHigh concentration acetic acid in the fermentation medium represses cell growth, metabolism and fermentation efficiency of Saccharomyces cerevisiae, which is widely used for cellulosic ethanol production. Our previous study proved that supplementation of zinc sulfate in the fermentation medium improved cell growth and ethanol fermentation performance of S. cerevisiae under acetic acid stress condition. However, the molecular mechanisms is still unclear. To explore the underlying mechanism of zinc sulfate protection against acetic acid stress, transcriptomic and proteomic analysis were performed. The changed genes and proteins are related to carbon metabolism, amino acid biosynthesis, energy metabolism, vitamin biosynthesis and stress responses. In a total, 28 genes showed same expression in transcriptomic and proteomic data, indicating that zinc sulfate affects gene expression at posttranscriptional and posttranslational levels.tration acetic acid in the fermentation medium represses cell growth, metabolism and fermentation efficiency of Saccharomyces cerevisiae, which is widely used for cellulosic ethanol production. Our previous study proved that supplementation of zinc sulfate in the fermentation medium improved cell growth and ethanol fermentation performance of S. cerevisiae under acetic acid stress condition. However, the molecular mechanisms is still unclear. To explore the underlying mechanism of zinc sulfate protection against acetic acid stress, transcriptomic and proteomic analysis were performed. The changed genes and proteins are related to carbon metabolism, amino acid biosynthesis, energy metabolism, vitamin biosynthesis and stress responses. In a total, 28 genes showed same expression in transcriptomic and proteomic data, indicating that zinc sulfate affects gene expression at posttranscriptional and posttranslational levels.
2022-05-19 | PXD015459 | Pride
Project description:We constructed non-flocculating strain PLY01 from industrial flocculating strain SPSC01. In the presence of 5.0 g/L acetic aicd stress, SPSC01 showed better growth and fermentation performance compared to PLY01. For probing mechanism of improved acetic acid tolerance induced by flocculation, we performed phosphoproteomic analysis to mine key proteins whose phosphorylation level has changed and regulation network.
2021-09-06 | PXD028217 | iProX
Project description:Genes whose expression correlated to the acetic acid tolerance in S. cerevisiae were identified by DNA microarray analysis. Gene expression profiles of two S. cerevisiae strains showing different levels of acetic acid tolerance were compared and an acetic acid tolerance-related gene chosen.
2012-08-30 | E-GEOD-36914 | biostudies-arrayexpress
Project description:TATA-Binding Protein Variants that Bypass the Requirement for Mot1 In Vivo
| PRJNA113205 | ENA
Project description:The modification of the The modification of the tolerance of xylose-fermenting yeast is an urgent issue for improving ethanol production. In this study, multiple genes involving in superoxide dismutase, glutathione biosynthesis, NADPH regeneration and acetic acid degradation were overexpressed using stress-induced promoters, which is selected from the transcriptome data. Stress-induced promoters were used to realize the feedback control of the tolerant genes, which can ultimately improve the tolerance and ethanol production. We reported the stress-induced promoters for overexpressing tolerant genes and increasing yeast tolerance in a feedback manner
2022-01-01 | GSE140075 | GEO
Project description:Genes whose expression correlated to the acetic acid tolerance in S. cerevisiae were identified by DNA microarray analysis.
2012-08-30 | GSE36914 | GEO
Project description:A new laboratory evolution approach to select for constitutive acetic-acid tolerance in Saccharomyces cerevisiae and identification of causal mutations
| PRJNA313456 | ENA
Project description:External application of acetic acid has been recently reported to enhance the survival to drought in plants such as Arabidopsis, rapeseed, maize, rice and wheat, but the effects of acetic acid application on increased drought tolerance in woody plants such as a tropical crop “cassava” remain elusive. A molecular understanding of acetic acid-induced drought avoidance in cassava will contribute to the development of technology that can be used to enhance drought tolerance without resorting to transgenic technology or advancements in cassava cultivation. In the present study, morphological, physiological and molecular responses to drought were analyzed in cassava after the treatment with acetic acid. Results indicated that the acetic acid-treated cassava plants had a higher level of drought avoidance than water-treated, control plants. Specifically, higher leaf relative water content, and chlorophyll and carotenoid levels were observed as soils dried out during the drought treatment. Leaf temperatures in acetic acid-treated cassava plants were higher relative to leaves on plants pretreated with water and the increase of ABA content was observed in leaves of acetic acid-treated plants, suggesting that stomatal conductance and the transpiration rate in leaves of acetic acid-treated plants decreased to maintain relative water contents and avoid drought. Transcriptome analysis revealed that the acetic acid treatment increased the expression of ABA signaling-related genes, such as OPEN STOMATA 1 (OST1) and protein phosphatase 2C; as well as drought response and tolerance-related genes, such as outer membrane tryptophan-rich sensory protein (TSPO), and heat shock proteins. Collectively, the external application of acetic acid enhances drought avoidance in cassava through the upregulation of ABA signaling pathway genes and several stress response- and tolerance-related genes. These data support the idea that adjustments of the acetic acid application to plants is useful to enhance drought tolerance in order to minimize the growth inhibition in the agricultural field.
2019-05-29 | GSE122140 | GEO