Project description:Triatoma infestans are insect triatomines and vectors of the protozoan Trypanosoma cruzi responsible for human Chagas' disease. Considering that T. cruzi multiplies inside the triatomine digestive tract (TDT), the analysis of the TDT protein profile is an essential step to understand TDT physiology during T. cruzi infection. To characterize the protein profile of TDT of T. infestans, a shotgun liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach was applied in this report.
Project description:Triatoma sordida salivary glands secrete a number of complex bioactive proteins during feeding that affect hemostatic and immunological systems from vertebrate hosts. In this study, salivary glands and saliva were collected from 5th instar nymphs and adult triatomines. Liquid chromatography tandem-mass spectrometry (LC-MS/MS) was used to identify T. sordida salivary proteins. A total of 133 proteins was identified. Lipocalins, Hemiptera specific families, CRiSP/Antigen-5 and Kazal-type protein inhibitors proteins match to 42.01%, 14.32%, 5.86% and 1.91% of the total spectrum count, respectively. These proteins are involved in several physiological roles, including anti-coagulant, immunity and anti-microorganism. Salivary proteins contribute to successful feeding.
Project description:Salivary multiprotein complexes from Triatoma infestans, Triatoma dimidiata, Dipetalogaster maxima, Rhodnius prolixus, and Rhodnius neglectus were identified by Blue-Native-PAGE (BN-PAGE) coupled with liquid chromatography tandem mass spectrometry.
Project description:The reduviid bug Triatoma dimidiata is an important Chagas disease vector widely distributed along Central America. This insect has great capability for domestic adaptation as the majority of specimens caught inside human dwellings or in peridomestic areas in Central America fed human blood. Within this context, exploring the salivary compounds that overcome host haemostatic and immune responses to assist blood-feeding is of great scientific interest. Our aim is to provide a deeper insight into T. dimidiata salivary gland molecules that may contribute to its haematophagic habit. We realized T. dimidiata salivary transcriptomic analysis and complemented it through proteomics, disclosing the set complexity of 119 secreted proteins. The large-scale approach used enriches the pharmacologically active molecules database and improves our knowledge about the complexity of salivary compounds from haematophagous vectors and their biological interactions.
Project description:Phytophthora spp. encode large sets of effector proteins and distinct populations of small RNAs (sRNAs). Reports suggest that pathogen-derived sRNAs can modulate the expression of plant defense genes. The experiments reported here were designed to shed light on impact of sRNAs in the potato-P. infestans interaction. We used the Argonaute or Ago1 from P. infestans tagged with GFP transformed into the 88069 strain to infect potato cv. Bintje plants. Collected leaf materials were used in co-immunoprecipitation experiments together with P. infestans harboring GFP (control GFP) and P. infestans mycelia grown on media (control mycelia). These three materials were sequenced at a Ion Proton platform. The reads length of 8-38 nt were adaptor-trimmed and mapped to the P. infestans genome and the Solanom tuberosum genome v4.04. Both P. infestans-associated and potato derived sRNAs were identified.