Genomics

Dataset Information

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Pyricularia oryzae


ABSTRACT: RNA-Seq Analysis of CNF2 and GPF1-dependent Genes in Magnaporthe oryzae 70-15

PROVIDER: PRJNA245759 | ENA |

REPOSITORIES: ENA

Dataset's files

Source:
Action DRS
SRR1265030.fastq.gz Fastqsanger.gz
SRR1265031.fastq.gz Fastqsanger.gz
SRR1265032.fastq.gz Fastqsanger.gz
SRR1265033.fastq.gz Fastqsanger.gz
SRR1265034.fastq.gz Fastqsanger.gz
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Publications

Systematic analysis of Zn2Cys6 transcription factors required for development and pathogenicity by high-throughput gene knockout in the rice blast fungus.

Lu Jianping J   Cao Huijuan H   Zhang Lilin L   Huang Pengyun P   Lin Fucheng F  

PLoS pathogens 20141009 10


Because of great challenges and workload in deleting genes on a large scale, the functions of most genes in pathogenic fungi are still unclear. In this study, we developed a high-throughput gene knockout system using a novel yeast-Escherichia-Agrobacterium shuttle vector, pKO1B, in the rice blast fungus Magnaporthe oryzae. Using this method, we deleted 104 fungal-specific Zn(2)Cys(6) transcription factor (TF) genes in M. oryzae. We then analyzed the phenotypes of these mutants with regard to gro  ...[more]

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