Project description:Human primary macrophages were treated with 1 µM staurosporine (STS) and profiled for small RNAs. Human primary macrophages were treated with 1 µM staurosporine (STS) for 0, 4 and 6h.
Project description:We report high-throughput profiling of gene expression from murine primary macrophages. We profile mRNA in control and endotoxin stimulated macrophages, and examine the effect of AHR ligand (SGA360) under inflammatory status. This study provides a framework for understanding transcriptional changes caused by SGA360 during activated inflammatory signaling .
Project description:We report that a high affinity, selective, small molecule Gpr120 agonist (cpdA), exerts potent anti-inflammatory effects on macrophages in vitro, and in obese mice in vivo. Gpr120 agonist treatment of high fat diet (HFD)/obese mice causes improved glucose tolerance, decreased hyperinsulinemia, increased insulin sensitivity and decreased hepatic steatosis. This suggests that Gpr120 agonists could become new insulin sensitizing drugs for the treatment of Type 2 diabetes and other human insulin resistant states in the future. Examination of effects of DHA and compound A on primary macrophages stimulated by LPS, 3 replicates for each condition
Project description:Fungal infections (mycoses) are common diseases of varying severity that cause problems especially to immunologically compromised people. During stimulatory conditions of infection and colonization, besides intensive intracellular response, human cells actively communicate on the intercellular level by secreting proteins with several mechanisms. The main immunostimulatory components of fungi are beta-glucans, fungal cell-wall components. Here, we have used high-throughput quantitative proteomics combined with bioinformatics to characterize and quantify vesicle-mediated protein release from beta-glucan-stimulated human primary macrophages. We show that β-glucan stimulation induces vesicle-mediated protein secretion. Proteomic study identified 540 distinct proteins from the vesicles including several receptors which have not been identified from vesicles before. Bioinformatics analysis strongly suggests a significant role for these proteins in transfer of biological information. Our current data provides an important resource on the protein composition of extracellular vesicles released from human macrophages upon fungal infection.
