Project description:As the critical gatekeeper for birth, prepartum remodeling of the cervix is associated with increased resident macrophages (MM-OM-^F), proinflammatory processes, and extracellular matrix degradation. This study tested the hypothesis that expression of genes unique to MM-OM-^Fs characterizes the prepartum from unremodeled nonpregnant cervix. Perfused cervix from prepartum day 21 postbreeding (D21) or nonpregnant (NP) rats, with or without MM-OM-^Fs, had RNA extracted and whole genome microarray analysis performed. By subtractive analyses, expression of 194 and 120 genes related to MM-OM-^Fs in the cervix from D21 rats were increased and decreased, respectively. In both D21 and NP groups, 158 and 57 MM-OM-^F genes were also more or less up- or down-regulated, respectively. Gene expression from four groups: D21 pregnant or non-pregnant rat cervix, with or without macrophages. Comparing the with and without macrophage groups can show genes that are expressed only in macrophages.
Project description:As the critical gatekeeper for birth, prepartum remodeling of the cervix is associated with increased resident macrophages (Mφ), proinflammatory processes, and extracellular matrix degradation. This study tested the hypothesis that expression of genes unique to Mφs characterizes the prepartum from unremodeled nonpregnant cervix. Perfused cervix from prepartum day 21 postbreeding (D21) or nonpregnant (NP) rats, with or without Mφs, had RNA extracted and whole genome microarray analysis performed. By subtractive analyses, expression of 194 and 120 genes related to Mφs in the cervix from D21 rats were increased and decreased, respectively. In both D21 and NP groups, 158 and 57 Mφ genes were also more or less up- or down-regulated, respectively.
Project description:In order to establish a rat embryonic stem cell transcriptome, mRNA from rESC cell line DAc8, the first male germline competent rat ESC line to be described and the first to be used to generate a knockout rat model was characterized using RNA sequencing (RNA-seq) analysis.
Project description:Inflammation is a key component of pathological angiogenesis. Here we induce cornea neovascularisation using sutures placed into the cornea, and sutures are removed to induce a regression phase. We used whole transcriptome microarray to monitor gene expression profies of several genes
