Genomics

Dataset Information

24

RNA-Seq: assessment of transcript level analysis tools


ABSTRACT: RNA-Seq: assessment of transcript level analysis tools

PROVIDER: PRJNA302625 | ENA |

REPOSITORIES: ENA

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Publications

Using Synthetic Mouse Spike-In Transcripts to Evaluate RNA-Seq Analysis Tools.

Leshkowitz Dena D   Feldmesser Ester E   Friedlander Gilgi G   Jona Ghil G   Ainbinder Elena E   Parmet Yisrael Y   Horn-Saban Shirley S  

PloS one 20160421 4


One of the key applications of next-generation sequencing (NGS) technologies is RNA-Seq for transcriptome genome-wide analysis. Although multiple studies have evaluated and benchmarked RNA-Seq tools dedicated to gene level analysis, few studies have assessed their effectiveness on the transcript-isoform level. Alternative splicing is a naturally occurring phenomenon in eukaryotes, significantly increasing the biodiversity of proteins that can be encoded by the genome. The aim of this study was t  ...[more]

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