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CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

ABSTRACT: CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

OTHER RELATED OMICS DATASETS IN: GSE78779

PROVIDER: PRJNA313513 | ENA |

REPOSITORIES: ENA

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CEL-Seq2: sensitive highly-multiplexed single-cell RNA-Seq

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2016-04-08 | GSE78779 | GEO

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Project description:We present Structure-seq2, which provides nucleotide-resolution RNA structural information in vivo and genome-wide. This optimized version of our original Structure-seq method increases sensitivity and data quality by minimizing formation of a deleterious by-product, reducing ligation bias, and improving read coverage. Structure-seq2 can employ a biotinylated nucleotide to facilitate the protocol. We have benchmarked Structure-seq2 on both mRNA and rRNA structure in rice (Oryza sativa) and apply Structure-seq2 to provide evidence of hidden breaks in chloroplast rRNA and a previously unreported N1-methyladenosine (m1A) in a nuclear-encoded rRNA.

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