Project description:Obese and lean type pig breeds show obvious differences in fat deposit and fatty acid composition. However, the underlying diversities in DNA methylation that related to the phenotypic differentiation have not been clarified. Here, we employed Landrace pigs (a leaner, Western breed) and Rongchang pigs (a fatty, Chinese breed) as models to survey genome-wide DNA methylation in the backfat adipose using a methylated DNA immunoprecopitation sequencing approch.We sequenced 6 methylated DNA immunoprecipitation (MeDIP) libraries, generated 38.27 gigabases (Gb) of methylated DNA immunoprecitation sequencing (MeDIP-seq) data from the six samples, representing about 15 times the size of the pig genome. About 88.89% of all reads could be aligned on the pig reference genome (Sus scrofa 10.2) using the SOAP2 software, and about 76.68% of reads had a unique genomic location. There was 483 different methylated regions (DMRs) located in genes promoter region, which are mainly involved in the processes of “olfactory and sensory activity” and “lipid metabolism”. This study provides a solid basis for exploring the epigenetic mechanisms of fat deposit and fatty acid composition, have benefit to promoting human health diet and improving pork quality and production efficiency.
Project description:Female domestic pigs were fed a 16-week Lean or Obese diet. Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq) We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in swine adipose-derived MSCs, and evaluated their reversibility using an epigenetic modulator, vitamin-C.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:Regulatory Mechanisms of Atrial Remodeling of Mitral Regurgitation Pigs This study enrolled 6 pigs (age: 18 months) and divided into three groups: mitral regurgitation pigs (MR) (n = 2; 2 males sacrificed 12 months after surgery), MR pigs treated with valsartan (MRV) (n = 2; 2 males age-matched to MR sacrificed 12 months after surgery), and normal control pigs (NC) (n = 2; 2 males age-matched to MR pigs). Valsartan (3.43 mg/kg/day), a type I angiotensin II receptor blocker, was administered from one week before surgery and then daily after surgery in the MRV group. We sought to systemically elucidate critical differences in the alteration of RNA expression pattern between the atrial myocardium of pigs with and without MR, and between the atrial myocardium of MR pigs with and without valsartan using high-density oligonucleotide microarrays and functional network enrichment analysis.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples