Project description:In order to gain insight into hepatic metabolic pathways and key transcripts affecting traits related to body composition we aimed to compare samples of pigs of two breeds, the âobeseâ German Landrace (DL) and the âleanâ Pietrain (Pi) obtained at prenatal stages (35, 63, and 91 days post conceptionem) and at adult age (180 days). In terms of number of genes regulated the most striking differences between DL and Pi were found at adult age with upregulation of key genes of lipid biosynthesis/metabolism pathways (FASN, ACSS2, ACACA) in obese DL pigs on the one hand and upregulation of genes of cell growth and/or maintenance, protein syntheses as well as cell proliferation pathways (PPARD, POU1F1, IGF2R) in lean Pi pigs on the other hand. Time course analysis of expression profiles of breed differences from foetal to adult stage and functional cluster analysis of the biological processes confirmed the trend of differences between genetically different obese and lean breeds. The highlighted classes of genes showed common breed-typical expression throughout prenatal development and at adult age. The transcriptional differences between obese and lean pigs involving lipid pathways and cell activity are already initiated during early prenatal development. The information about genetic differences between obese and lean pigs reveals a number of functional candidate genes for traits related to obesity and leaness. Porcine liver from three prenatal stages (35, 63, and 91 day post conceptionem, dpc) plus adult age (180 days) of 10 animals of each of the breeds DL and Pi, which differ in body composition, were collected. The hepatic gene expression patterns of DL and Pi breeds were compared at each of the four stages of development. Three or four biological replicate microarray hybridisations were performed for each stage with DL pools labelled with Cy5 and Pi pools labelled with Cy3.
Project description:Female domestic pigs were fed a 16-week Lean or Obese diet. Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq) We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in swine adipose-derived MSCs, and evaluated their reversibility using an epigenetic modulator, vitamin-C.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:In order to gain insight into hepatic metabolic pathways and key transcripts affecting traits related to body composition we aimed to compare samples of pigs of two breeds, the “obese” German Landrace (DL) and the “lean” Pietrain (Pi) obtained at prenatal stages (35, 63, and 91 days post conceptionem) and at adult age (180 days). In terms of number of genes regulated the most striking differences between DL and Pi were found at adult age with upregulation of key genes of lipid biosynthesis/metabolism pathways (FASN, ACSS2, ACACA) in obese DL pigs on the one hand and upregulation of genes of cell growth and/or maintenance, protein syntheses as well as cell proliferation pathways (PPARD, POU1F1, IGF2R) in lean Pi pigs on the other hand. Time course analysis of expression profiles of breed differences from foetal to adult stage and functional cluster analysis of the biological processes confirmed the trend of differences between genetically different obese and lean breeds. The highlighted classes of genes showed common breed-typical expression throughout prenatal development and at adult age. The transcriptional differences between obese and lean pigs involving lipid pathways and cell activity are already initiated during early prenatal development. The information about genetic differences between obese and lean pigs reveals a number of functional candidate genes for traits related to obesity and leaness. Keywords: pig, oligo-microarray, prenatal liver expression, adult liver expression, obesity, leaness
Project description:Regulatory Mechanisms of Atrial Remodeling of Mitral Regurgitation Pigs This study enrolled 6 pigs (age: 18 months) and divided into three groups: mitral regurgitation pigs (MR) (n = 2; 2 males sacrificed 12 months after surgery), MR pigs treated with valsartan (MRV) (n = 2; 2 males age-matched to MR sacrificed 12 months after surgery), and normal control pigs (NC) (n = 2; 2 males age-matched to MR pigs). Valsartan (3.43 mg/kg/day), a type I angiotensin II receptor blocker, was administered from one week before surgery and then daily after surgery in the MRV group. We sought to systemically elucidate critical differences in the alteration of RNA expression pattern between the atrial myocardium of pigs with and without MR, and between the atrial myocardium of MR pigs with and without valsartan using high-density oligonucleotide microarrays and functional network enrichment analysis.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples