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SMAD2/3 are redirected to novel sites in MCF10A MII after prolonged TGFβ stimulation

ABSTRACT: SMAD2/3 are redirected to novel sites in MCF10A MII after prolonged TGFβ stimulation

PROVIDER: PRJNA326978 | ENA |

REPOSITORIES: ENA

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JUNB is a critical AP1 component for SMAD2/3 binding after TGFβ stimulation [RNA-seq]

Project description:We performed SMAD2/3 ChIP-seq analysis in MCF10A MII cells. To validate whether the changes in SMAD2/3 binding to the genome indeed resulted in changes in target gene expression, we performed RNA-seq transcriptome analysis after short and long periods of TGFβ stimulation (0, 1.5h and 16h) in MII cells. In addition, we revealed that JUNB is a critical AP1 component for SMAD2/3 binding after TGFβ stimulation. To assess the significance of JUNB for TGFβ-SMAD-target genes on a genome-wide scale, we also performed RNA-seq transcriptome analysis in JUNB-knock-downed MII cells.

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Project description:We report that SMAD2/3 are redirected to novel binding sites on the genome after prolonged TGFβ stimulation.

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SMAD2/3 are redirected to novel sites in MCF10A MII after prolonged TGFβ stimulation

Project description:This SuperSeries is composed of the SubSeries listed below.

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