Project description:Small and long RNAs profiling of developing oocytes and early embryos in wild-type and Pld6-deficient golden hamster females, as well as profiling of testis of wild-types and Pld6-mutents
Project description:We profiled transcriptomes from Cnot6l deadenylase knock-out mouse GV oocytes, MII eggs and 1-cell zygotes in order to analyse its function during the oocyte-to-embryo (OET) transition. Transcriptome of wild-type golden hamster GV oocytes was also profiled.
Project description:Transcriptional profiling of male Syrian hamster testes, comparing control animals with those that have had a pinealectomy. Keywords: transcriptional profiling
Project description:Transcriptional profiling of male Syrian hamster testes, comparing control animals with those that have had a pinealectomy. Keywords: transcriptional profiling Two-condition experiment, Control vs pinealectomised animals
Project description:We have mapped regulatory loci for nearly all protein coding genes in the mammalian genome using microarray measurements from a panel of mouse/hamster radiation hybrids. Experiment Overall Design: 99 RH cells with biological replicates (198 total) and biological replicates of mouse brain RNA vs. hamster brain RNA and mouse liver RNA vs. hamster liver RNA (4 total)
Project description:We used SLIC-CAGE to map transcriptional start sites (TSSs) of P14 WT and P14 Tbpl2-/- mutant mouse oocytes. Comparison of WT and Tbpl2-/- oocytes demonstrates that Tbpl2 guides transcriptional start site selection in the growing oocyte. This TSS selection is different compared to the canonical somatic type of TSS selection and depends on TATA-like elements as core promoter motifs, recognised by Tbpl2.