Project description:Various RNAs have been shown to elicit innate immunity by activating endosomal PRRs such as Toll-like receptors 3/7/8 (TLR3/7/8), and cytosolic PRRs (e.g., retinoic acid-inducible gene I (RIG-I), melanoma differentiation-associated protein 5 (MDA5)). Such innate immunity provides cytokines and co-stimulation signals that are essential for adaptive immunomodulation.
Project description:Various RNAs have been shown to elicit innate immunity by activating endosomal PRRs such as Toll-like receptors 3/7/8 (TLR3/7/8), and cytosolic PRRs (e.g., retinoic acid-inducible gene I (RIG-I), melanoma differentiation-associated protein 5 (MDA5)). Such innate immunity provides cytokines and co-stimulation signals that are essential for adaptive immunomodulation.
Project description:The importance of unanchored Ub in innate immunity has been shown only for a limited number of unanchored Ub-interactors. We investigated what additional cellular factors interact with unanchored Ub and whether unanchored Ub plays a broader role in innate immunity. To identify unanchored Ub-interacting factors from murine lungs, we used His-tagged recombinant poly-Ub chains as bait. These chains were mixed with lung tissue lysates and protein complexes were isolated with Ni-NTA beads. Sample elutions were subjected to mass spectrometry (LC-MSMS) analysis.
Project description:Bronchial epithelial cells represent the first line of defense against invading airborne pathogens. They are important contributors to innate mucosal immunity and provide a variety of anti-microbial effectors. To investigate the role of epithelial cells upon infection of airway pathogens, we stimulated BEAS-2B cells for 4 h with UV-inactivated bronchial pathogens including Staphylococcus aureus, Pseudomonas aeruginosa and Respiratory Syncitial Virus (RSV) that among other receptors can strongly activate TLR2, TLR4 and TLR3, respectively. Experiment Overall Design: All conditions were done in triplicates except for Staphylococcus aureus, were two replicates were done. As a control, unstimulated BEAS-2B were used. Altogether 11 arrays were hybridized.
Project description:A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes.
