Project description:The finding that small non-coding RNAs (sRNAs) can affect cellular processes by regulating gene expression had a significant impact on biology research and clinical diagnosis. Yet, the ability to quantify and profile sRNAs, especially miRNAs, using high-throughput sequencing is especially challenging because of their repetitive nature. We have developed QsRNA-seq a method for preparation of sRNA libraries for high-throughput sequencing that overcomes this difficulty by enabling separation of fragments differing only by 20nt in length and implementing barcode and unique molecular identifiers for multiplexing and accurate quantification. We show that this method gives very accurate, comprehensive and reproducible results. Using QsRNA-seq to study the miRNA repertoire in C. elegans embryo and L4 larval developmental stages, we found many miRNAs that are expressed in a developmental-specific manner. Interestingly, when profiling miRNA length, we found that miRNAs 23-nt long are predominantly expressed in L4 developmental stage and not embryo.
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: high-throughput 454 sequencing
Project description:High-throughput sequencing of small RNAs from HeLa cells Keywords: high-throughput Solexa sequencing Small RNAs were sequenced from HeLa cells
Project description:High-throughput sequencing of mixed-stage Caenorhabditis elegans small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: high-throughput 454 sequencing
Project description:High-throughput sequencing of Arabidopsis thaliana endogenous small RNAs by 454 pyrosequencing. Keywords: high-throughput sequencing
Project description:High-throughput sequencing of small RNAs from Xenopus tropicalis (adult liver, adult skin, oocytes stage I, II, III, IV, V, VI). total RNA, ~18-42 nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH