Project description:Whole genome resequencing of extreme phenotypes in collared flycatchers highlights the difficulty of detecting quantitative trait loci in natural populations
Project description:Detecting and monitoring tumor clonal evolution in the setting of measurable residual disease is technically difficult due to 1) high sequencing background due to limited numbers of disease cells, 2) loss of tumor genetic material due to the need for high quality purified DNA, and 3) resource and bioinformatics intensive requirements of traditional next generation sequencing techniques. Limited Cell Fluorescence Activated Cell-sort Sequencing (LC-FACSeq), is a fast, reproducible, and cost effective amplicon based sequencing technique that does not require genomic material isolation. It can be used in the context of just a few hundred tumor cells and is easily generalization for monitoring clonal evolution in various different malignancies.
Project description:In brief, we have applied a novel and unbiased approach to identify distinct and dynamic cell populations, including an in-depth analysis of the fibroblast population, in lung fibrosis. We found that while fibroblast number did not increase after injury, a population of activated fibroblasts can be identified in the fibrotic mouse lung. Using expression profiles of 1,945 lung fibroblasts we redefine the molecular characteristics of activated fibroblasts, finding that they are not a separate population but rather exhibit a similar, yet amplified, gene expression pattern to non-activated cells.