Single-cell RNA sequencing of vascularized brain organoids
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PROVIDER: PRJNA427709 | ENA |
REPOSITORIES: ENA
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Project description:Organoids have been widely used as unique models of human brain development and disorders. However, the lack of vasculatures in brain orgnoids limits their application in the study of brain vasculature development and diseases. Here, we described to the generation of vascularized brain organoids (VBOrs) with different brain regions from human embryonic stem cells. The goals of this study are to analyze the cell populations of the new model of vasularized brain organoids cultured from human embryonic stem cells (H9). We found that VBOrs contain variant brain cells inculding neural progenitors, neuronal cells, astrocytes, sparse endothelial cells, and pericytes. The new model of VBOrs should be valuable for addressing questions between brain vasculatures and neural cells.
2017-12-28 | GSE108571 | GEO
Project description:Organoids were generated from H9 cells. Single cells were sorted from 4-month-old brain organoids differentiated using the telencephalon organoids protocol.
2023-07-10 | E-MTAB-13144 | biostudies-arrayexpress
Project description:Modeling the processes of neuronal progenitor proliferation and differentiation to produce mature cortical neuron subtypes is essential for the study of human brain development and the search for potential cell therapies. We demonstrated a novel paradigm for the generation of vascularized organoids (vOrganoids) consisting of typical human cortical cell types and a vascular structure for over 200 days as a vascularized and functional brain organoid model. The observation of sEPSCs (spontaneous excitatory postsynaptic currents), sIPSCs (spontaneous inhibitory postsynaptic currents) and bidirectional electrical transmission indicated the presence of chemical and electrical synapses in vOrganoids. More importantly, single-cell RNA-sequencing analysis illustrated that vOrganoids exhibited robust neurogenesis and that cells of vOrganoids differentially expressed genes related to blood vessel morphogenesis. The transplantation of vOrganoids into the mouse S1 cortex resulted in the construction of functional human-mouse blood vessels in the grafts that promoted cell survival in the grafts. This vOrganoid culture method could not only serve as a model to study human cortical development and explore brain disease pathology but also provide potential prospects for new cell therapies for nervous system disorders and injury.
2020-06-04 | GSE131094 | GEO
Project description:Single-cell RNA sequencing of 4-month-old telencephalon organoids. Organoids were generated from cells infected with pooled lentivirus library. The lentiviral library delivers dual-gRNAs targeting 36 high-risk ASD genes in parallel. Organoids were dissociated after 4 months of in vitro differentiation and maturation. Single cells were sorted and subjected to 10X genomics 3' kits.
2023-07-09 | E-MTAB-13148 | biostudies-arrayexpress
Project description:To study the effect of GLI3 knockout on early brain organoid development, we collected single-cell multiome data from 18 day old brain organoids
2022-08-25 | E-MTAB-12002 | biostudies-arrayexpress
Project description:The human brain has changed dramatically from other primate species, but the genetic and developmental mechanisms behind the differences remains unclear. Here we used single cell RNA sequencing based on 10X technology to explore temporal transcriptomic dynamics and cellular heterogeneity in cerebral organoids derived from human and non-human primates chimpanzee and rhesus macaque stem cells. Using cerebral organoids as a proxy of early brain development, we detect a delayed pace of human brain development relative to the other two primate species. Additional human-specific gene expression patterns resolved to different cell states through progenitors to neurons are also found. Our data provide a transcriptomic cell atlas of primate early brain development, and illustrate features that are unique to humans.
2019-12-30 | E-MTAB-7552 | biostudies-arrayexpress
Project description:The human brain has changed dramatically from other primate species, but the genetic and developmental mechanisms behind the differences remains unclear. Here we used single cell RNA sequencing based on 10X technology to explore temporal transcriptomic dynamics and cellular heterogeneity in cerebral organoids derived from human and non-human primates chimpanzee and rhesus macaque stem cells. Using cerebral organoids as a proxy of early brain development, we detect a delayed pace of human brain development relative to the other two primate species. Additional human-specific gene expression patterns resolved to different cell states through progenitors to neurons are also found. Our data provide a transcriptomic cell atlas of primate early brain development, and illustrate features that are unique to humans.
2019-10-04 | E-MTAB-8234 | biostudies-arrayexpress
Project description:Human brain organoids are three-dimensional cultures that recapitulate in vivo cell diversity and organization, which provide a novel source for transplantation therapies of neurological disorders. However, some remaining technical problems including surgical lesions which still limit the application of brain organoid transplantation. Here instead of transplanting mature organoids, we performed development of organoids in vivo (IVD-organoids) by injecting small early organoids into the adult mice corpus striatum. Single-cell transcriptome analysis suggested that IVD-organoids contain pericyte-like and hippocampal cells. Similar to previous studies in cerebral organoid transplantation, IVD-organoids also showed reduced cellular stress and death. We further demonstrated that more choroid plexus (ChP) cells were generated in IVD-organoids, which were important for maintaining brain homeostasis. Together, our study provides a novel method that allows in vivo generation of human brain organoids, which may serve as a potential cell therapy for neurological disease involving different brain regions.
2022-03-15 | GSE165975 | GEO
Project description:Characterization of human stem cell-derived microglia (hMG) that develop within vascularized human brain organoids under physiological conditions in vivo. We isolated tdT+/CD45+ hMG from five animals and three time points (6, 12 and 24 weeks post transplantation) using Fluorescence-activated cell sorting (FACS), following a strictly controlled ex vivo isolation procedure as previously described (Gosselin et al., 2017) and profiled those cells using single cell RNA sequencing.
2023-05-11 | E-MTAB-11522 | biostudies-arrayexpress
Project description:Vascularized human cortical organoids (vOrganoid) model cortical development in vivo
| PRJNA542649 | ENA