Project description:In order to gain insight into abundance of transcripts in the presence and absence of m6A, we performed an mRNA sequencing expirement in both mta and Col-0 plants.
Project description:In order to assess the possible effects of m6A on miRNA biogenesis we performed small RNA (sRNA) sequencing to compare their levels in WT Col-0 plants to those in mutant lines with severely reduced m6A levels. The reduced mta line contains MTA cDNA under the ABI3 promoter in MTA null mutant background (ABI3:MTA) and ABI3 being an embryo specific promoter rescues the embryo lethal phenotype of MTA knockout while keeping the levels of MTA in mature plants severely low34. sRNAs isolated from 4 week old WT and mta plants were used to make libraries that were sequenced using HiSeq® (Illumina) and the results were analyzed using NOISeq package in R-Studio.
Project description:In order to gain insight into relative stability of transcripts in plants that lacked m6A, we performed global mapping of uncapped and cleaved transcripts
Project description:In order to investigate the influence of m6A on RNA secondary-structure and RNA-protein interactions, we performed protein-interaction profile sequencing (PIP-seq)
Project description:We performed m6A-RNA immunoprecipitation followed by sequencing (m6A-IP Seq) using libraries produced with unfragmented ployA+ RNA from 4 week old WT Col-0 and mta plants.
Project description:In order to gain insight into abundance of transcripts in the presence and absence of m6A, we performed an mRNA sequencing expirement in both mta and Col-0 plants.
Project description:Lipid droplets from 6 week old Arabidopsis leaves were purified on sucrose gradient. The abundance of the proteins in the lipid droplet fraction was established by label free and compared to soluble, membrane and plastoglobule fractions, and total leaf extract in order to identify the proteins specifically enriched in lipid droplets. Three independent biological replicates, from three independent cultures were performed. For each biological replicates, three preparations of lipid droplets (and other fractions) within each of the three independent cultures were obtained.