Project description:ChIP-seq of histone H3 was performed on two biological replicates of the same W303 strain of wild-type cells and two independent isolates of each of isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>
Project description:We report the nuclear Gcn5/α-KGDH complex plays vital role in histone H3 K79 succinylation. By obtaining over 731 million reads from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of histone H3 K79 succinylation, Gcn5, and α-KGDH in U251 cells.
Project description:MNase-seq was performed in WT, isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: MNase_<growth condition>_<genotype-replicate>_<strain name>_<units MNase>
Project description:ChIP-seq of FLAG-tagged Pol2 (subunit of DNA Polymerase epsilon) was performed on two independent isolates of each of WT, isw2∆, nhp10∆, and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype-replicate>_<strain name>
Project description:In budding yeast, telomeres and the mating type (HM) loci are found in a heterochromatin-like silent structure initiated by Rap1 and extended by the interaction of Sir (Silencing Information Regulator) proteins with histones. Binding data demonstrate that both the H3 and H4 N terminal domains required for silencing in vivo interact directly with Sir3 and Sir4 in vitro. The role of H4 lysine 16 deacetylation is well established in Sir3 protein recruitment, however that of the H3 N terminal tail has remained unclear. In order to characterize the role of H3 in silent chromatin formation and compare it to H4 we have generated comprehensive high resolution genome-wide binding maps of heterochromatin proteins. We find that H4 lysine 16 deacetylation is required for the recruitment and spreading of heterochromatin proteins at all telomeres and HM loci. In contrast the H3 N terminus is required for neither recruitment nor spreading of Sir proteins. Instead, deletion of the H3 tail leads to increased accessibility within heterochromatin of an ectopic bacterial dam methylase and the decreased mobility of an HML heterochromatic fragment in sucrose gradients. These findings indicate an altered chromatin structure. We propose that Sir proteins recruited by the H4 tail then interact with the H3 tail to form a higher order silent chromatin structure.
Project description:ChIP-seq of FLAG-tagged RPA190 (subunit of RNA Polymerase I) or FLAG-tagged RPO31 (subunit of RNA Polymerase III) was performed on WT and isw2∆ nhp10∆ cells. Sample naming convention: <ChIP target>_<growth condition>_<genotype>_<strain name>