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Expression data from rat insulinoma INS-1 cells under glucotoxic condition

ABSTRACT: Expression data from rat insulinoma INS-1 cells under glucotoxic condition

PROVIDER: PRJNA454198 | ENA |

REPOSITORIES: ENA

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Expression data from rat insulinoma INS-1 cells under glucotoxic condition

Project description:Glucotoxicity of INS-1 cells stimulation by chronic high glucose. We used microarrays to detail the global programme of gene expression underlying cellularization and identified distinct classes of up and down-regulated genes during this process.

2018-05-01 | GSE113828 | GEO

Transcription profiling of rat insulinoma INS-1 cell lines (FLP-In T-REx)

Project description:Four rat insulinoma cell lines (INS-1) were generated that contain a FRT site for FLP recombinase mediated site specific integration of specific genes. In addition, the cell lines contain the tetracycline repressor allowing tetracycline (tet) induction of the introduced gene. By comparing the gene expression profiles of uninduced and tet-induced cells, target genes of introduced transcription factors or other signal tranduction molecules can be identified. The expression profiles of the parental INS-1 cell line and the four derivatives were determined on a RAE230A array, showing that the highly differentiated phenotype of the parental cell line was maintained in the clones.

2008-06-11 | E-GEOD-1591 | biostudies-arrayexpress

Transcription profiling of rat insulinoma line INS-1 to identify targets of HNF1b mutants in INS-1

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2008-06-11 | E-GEOD-1590 | biostudies-arrayexpress

sRNA sequencing of miRNAs exported to HDL from INS-1 rat insulinoma cells

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Gene expression in response to short and long term cAMP stimulation in the INS-1 insulinoma cell line

2011-11-22 | GSE31736 | GEO

Gene expression in response to short and long term cAMP stimulation in the INS-1 insulinoma cell line

Project description:Long term exposure to incretin hormones is known to have salutory effects on beta cell function and viability. While short-term cAMP induction is known to have a signature CREB-CRTC target gene response, the long-term effects of cAMP on beta cell gene expression are less well understood. We used rat microarray analysis to compare the genome-wide gene expression response to short-term (2 hours) and long-term (16 hours) stimulations of the cAMP agonist forskolin in INS-1 insulinoma cells. INS-1 cells were exposed to forskolin for 2 or 16 hours in RPMI medium containing 10% serum. Control samples wer incubated for the same time without forskolin. Extracted RNA was used for hybridization on Affymetrix rat 1.0 st gene arrays.

2011-11-22 | E-GEOD-31736 | biostudies-arrayexpress

Transcription profiling of rat insulinoma (INS-1 derived) cell lines that vary in their glucose responsiveness

Project description:The aim of this study was to identify the gene expression changes associated with glucose responsiveness in Rat INS-1 derived cell lines. RNA was prepared from cell lines which were shown to be highly glucose responsive and also lines which were shown to be poorly glucose responsive. Dr. Chris Newgard's lab quantified the RNA and sent it frozen in water. 3 biological replicates per condition were sent for the following conditions: (i) 832/13 and 833/15, robust glucose responsiveness; (ii)832/1 and 832/2 showed poor glucose responsiveness.

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INS-1 cell lines (FLP-In T-REx)

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Gene expression changes in response to doxycyline-induced expression of proinsulin (C96Y)-GFP in an INS-1 insulinoma cell line

Project description:A doxycyline-inducible INS-1 insulinoma cell line expressing proinsulin (C96Y)-GFP was engineered. Addition of doxycyline causes the production of the proinsulin (C96Y)-GFP, which is retained in the endoplasmic reticulum. This study analyzes the gene expression changes that occur after doxycyline-induced expression of proinsulin (C96Y)-GFP for 24h, 48h and 5 days. Expression changes were compared between control un-induced cells and cells treated with doxycyline. Three replicates (experiments) were performed for each time point. Stable mutant insulin-expressing insulinoma cells were treated or not with doxycyline for 24h, 48h or 5 days and RNA was extracted for expression analysis.

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sRNA sequencing of miRNAs exported to HDL from INS-1 rat insulinoma cells

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