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CircRNA-Seq Analysis for Sciatic Nerve Injury of Rat

ABSTRACT: CircRNA-Seq Analysis for Sciatic Nerve Injury of Rat

PROVIDER: PRJNA474515 | ENA |

REPOSITORIES: ENA

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CircRNA-Seq Analysis for Sciatic Nerve Injury of Rat

Project description:Here, we established a rat sciatic nerve injury model and investigated the circRNA expression profiles by Next-generation sequencing. In all, 7222 distinct circRNA candidates were found in these tissues and 4942 of these circRNAs contained at least two unique back-spliced reads. On the basis of expression of circRNA analysis, we found 131 circRNAs to be significantly (p-value<=0.05, Fold-change>=2) differently expressed in injured sciatic nerve tissues compared with matched normal tissues. All these circRNAs are derived from 21 rat chromosomes.

2020-01-01 | GSE115315 | GEO

Gene expression from aged and young sciatic nerve after nerve injury

Project description:Sciatic nerve ligation was performed on cohorts of 2-month and 24-month old animals. Resulting gene-expression data were generated from sciatic nerve 1 and 4 days after injury compared to naïve animals. Results show differences in sciatic nerve responses with normal aging. Total RNA taken from sciatic nerves from 2-month and 24-month old animals at either day 0, 1 and 4 after sciatic nerve crush injury.

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Transcription profiling of rat adult and neonate dorsal horn after sciatic nerve injury

Project description:As rats do not develop neuropathic pain like hypersensitivity as neonates post nerve injury but do as adults we have used these arrays to help define the processes involved in this process. Rat spinal cord (ipsilateral dorsal horn) was assayed 7 days post SNI injury to the sciatic nerve relative to sham injury. Two age groups of animals were tested Neonates (P10) and Adult (8-12wks). Experiment Overall Design: Six biologically indepenedent arrays were hybridized per assay point. Dorsal horn total RNA was prepared using standard Affymetrix protocols. Affymetrix Rat Expression 230A array used.

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Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [proximal sciatic nerve]

Project description:We applied Solexa sequencing technology to identify rat microRNA genes in proximal sciatic nerve following sciatic nerve resection. Using Solexa sequencing, computational analysis and Q-PCR verification, 93 novel miRNAs in rats were discovered and identified, of which 42 novel miRNAs were first reported in proximal sciatic nerve of rat and 51 novel miRNAs were produced at days 1, 4, 7 and 14 after sciatic nerve resection. These data provide an important resource relating to the role and regulation of miRNAs for future studies relating to peripheral nerve injury and regeneration. Keywords: Small RNA sequencing 18-30 nt small RNAs from proximal sciatic nerve of 30 Thirty Sprague-Dawley (SD) rats were sequenced at one Solexa lane

2012-05-06 | E-GEOD-26753 | biostudies-arrayexpress

Solexa sequencing of small RNAs in Sprague-Dawley (SD) rat [proximal sciatic nerve]

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Gene expression from aged and young sciatic nerve after nerve injury

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Altered microRNA expression in sciatic nerve following injury in rat model (0d-14d)

Project description:We used microarrays to distinguish the miRNA expression differences among different time points in sciatic nerve after injury.

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Rat sciatic nerve axoplasm proteome is enriched with ribosomal proteins during regeneration processes.

Project description:Analysis of extruded axoplasm proteomes from adult rat sciatic nerves in vivo by Label Free Quantitative Proteomics (LFQ) and comparison of normal and injury (18 hours post complete nerve transection) conditions. A combination of BONCAT and MS/MS were also performed to analyze the top axonal newly synthetized proteins in vivo.

2020-11-18 | MSV000086475 | MassIVE

Adult and Neonatal dorsal horn gene expression 7 day post sciatic nerve SNI injury

Project description:As rats do not develop neuropathic pain like hypersensitivity as neonates post nerve injury but do as adults we have used these arrays to help define the processes involved in this process. Rat spinal cord (ipsilateral dorsal horn) was assayed 7 days post SNI injury to the sciatic nerve relative to sham injury. Two age groups of animals were tested Neonates (P10) and Adult (8-12wks). Keywords: Two way analysis of differential regulation

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In vivo analysis of camptothecin effect in primary DRG neuron after sciatic nerve injury

Project description:Analysis of gene expression in injured primary DRG with or without camptothecin (CPT) treatment after sciatic nerve crushing may help us identify critical molecular pathways related to axon regeneration. We performed RNA-sequencing of (i) Naive primary DRG tissues without injury, (ii) Primary DRG tissues with vehicle treatment different time-points (18, 24, 36 hours) after sciatic nerve injury, and (iii) Primary DRG tissues with camptothecin treatment different time-points (18, 24, 36 hours) after sciatic nerve injury.

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