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ChIP-Chip tiling array for H3k27ac in mouse MV+ (lens epithelium) and RAG cells ( renal adenocarcinoma) over panel of genes including Pax6, Sox2, Otx2, Sox9.

ABSTRACT: ChIP-Chip tiling array for H3k27ac in mouse MV+ (lens epithelium) and RAG cells ( renal adenocarcinoma) over panel of genes including Pax6, Sox2, Otx2, Sox9.

PROVIDER: PRJNA489871 | ENA |

REPOSITORIES: ENA

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ChIP-Chip tiling array for H3k27ac in mouse MV+ (lens epithelium) and RAG cells ( renal adenocarcinoma) over panel of genes including Pax6, Sox2, Otx2, Sox9.

Project description:Enhancer identification using H3k27ac ChIP-ChIP in mouse MV+ and RAG, using custom tilling array covering a 66 Mb region around Pax6 (Chr2:75,000,000-141,000,000) mm9. Additional loci and regions, Sox2 (chr3:32,000,000-37,000,000), Otx2 (chr14:48,500,000-50,500,000), Rax (chr18:65,500,000-68,000,000), Hba a-globin (chr11:32,050,000-32,450,000), Six3 (chr17:85,200,000-86,700,000), Sox1 (chr8:11,800,000-12,800,000), Mab21L2 (chr3:85,850,000-86,850,000), Bmp4 (chr14:46,500,000-47,500,000), NeuroD1 (chr2:79,050,000-79,600,000), Prox1 (chr1:191,700,000-192,700,000), Hmx1 (chr5:34,700,000-36,200,000), H19-Igf2 (chr7:149500000-150500000), Sox9 (chr11:110000000-116000000).

2018-10-18 | GSE119659 | GEO

ChIP-Chip tiling array for CTCF and Rad21 in mouse β-TC3 cells (pancreatic insulinoma) MV+ (lens epithelium) and RAG cells (renal adenocarcinoma) over panel of genes including Pax6, Sox2, Otx2, Sox9.

Project description:CTCF and Rad21 ChIP-ChIP in mouse pancretic cells, pancreatic β-TC3 cells, lens epithelium MV+ cells and renal adenocarcinoma RAG cells, using custom tilling array covering a 66 Mb region around Pax6 (Chr2:75,000,000-141,000,000) mm9. Additional loci and regions, Sox2 (chr3:32,000,000-37,000,000), Otx2 (chr14:48,500,000-50,500,000), Rax (chr18:65,500,000-68,000,000), Hba a-globin (chr11:32,050,000-32,450,000), Six3 (chr17:85,200,000-86,700,000), Sox1 (chr8:11,800,000-12,800,000), Mab21L2 (chr3:85,850,000-86,850,000), Bmp4 (chr14:46,500,000-47,500,000), NeuroD1 (chr2:79,050,000-79,600,000), Prox1 (chr1:191,700,000-192,700,000), Hmx1 (chr5:34,700,000-36,200,000), H19-Igf2 (chr7:149500000-150500000), Sox9 (chr11:110000000-116000000).

2018-10-18 | GSE119658 | GEO

Characterisation by Next generation Capture-C of Pax6 locus in three mouse cell lines (MV+, RAG and β-TC3 cells).

Project description:NG-Capture-C profiling of chromatin interactions (using DpnII 3C and Oligo nucleotide sequence capture followed by Next generation sequencing) of enhancers, promoters and CTCF/Rad21 binding sites throughout a ~600kb region of the mouse Pax6 locus in three expression states, High (β-TC3), On (MV+) and OFF (RAG). β-TC3 cell are a mouse pancreatic β cell derived from mouse insulinoma, MV+ a lens epithelium cell line and RAG a mouse kidney adenocarcinoma cell line.

2018-10-18 | GSE120666 | GEO

Characterisation of three mouse cell lines by ATAC-seq (MV+, RAG and β-TC3 cells).

Project description:Chromatin accessibility profiling using ATAC-seq to study and identify novel Cis-regulatory elements of MV+ a lens epithelium cell line, RAG a mouse kidney adenocarcinoma cell line and β-TC3 cell a mouse pancreatic β cell, derived from mouse insulinoma.

2018-10-18 | GSE119656 | GEO

ChIP-Chip tiling array for Rad21 in RAG cells (renal adenocarcinoma) over mouse Pax6 gene region. Agilent ChIP-chip, Agilent 4x180K.

Project description:Rad21 ChIP-ChIP in renal adenocarcinoma RAG cells, using custom tilling array covering Pax6 mm9 (Chr2:103500000-107499954) mm9. This study is part of a large data set study gene regulotry mechnism in the Pax6 locus and complements other ChIP-ChIP data.

2018-10-18 | GSE120665 | GEO

Project description:ChIP-Chip tiling array for CTCF and Rad21 in mouse β-TC3 cells (pancreatic insulinoma) MV+ (lens epithelium) and RAG cells (renal adenocarcinoma) over panel of genes including Pax6, Sox2, Otx2, Sox9.

| PRJNA489872 | ENA

ChIP-Chip tiling array for H3k27ac in mouse β-TC3 cells over panel of genes including Pax6, Sox2, Otx2, Sox9.

Project description:Enhancer identification using H3k27ac ChIP-ChIP in mouse β-TC3 cells, using custom tilling array covering a 66 Mb region around Pax6 (Chr2:75,000,000-141,000,000) mm9. Additional loci and regions, Sox2 (chr3:32,000,000-37,000,000), Otx2 (chr14:48,500,000-50,500,000), Rax (chr18:65,500,000-68,000,000), Hba a-globin (chr11:32,050,000-32,450,000), Six3 (chr17:85,200,000-86,700,000), Sox1 (chr8:11,800,000-12,800,000), Mab21L2 (chr3:85,850,000-86,850,000), Bmp4 (chr14:46,500,000-47,500,000), NeuroD1 (chr2:79,050,000-79,600,000), Prox1 (chr1:191,700,000-192,700,000), Hmx1 (chr5:34,700,000-36,200,000), H19-Igf2 (chr7:149500000-150500000), Sox9 (chr11:110000000-116000000).

2018-08-01 | GSE116805 | GEO

ChIP-Chip tiling array for Rad21 in RAG cells (renal adenocarcinoma) over mouse Pax6 gene region. Agilent ChIP-chip, Agilent 4x180K.

Project description:ChIP-Chip tiling array for Rad21 in RAG cells (renal adenocarcinoma) over mouse Pax6 gene region. Agilent ChIP-chip, Agilent 4x180K.

| PRJNA494026 | ENA

RNA-seq profiling of mouse transformed culture cells as Pax6 locus cell models

Project description:Transcriptome profiling using RNA-seq of MV+, a mouse lens epithelium cell line expressing Pax6 and RAG renal adenocarcinoma cell line which does not express Pax6.

2018-10-18 | GSE119660 | GEO

Otx2 controls regional patterning within the MGE and regional identity of the septum [ChIP-Seq]

Project description:The Otx2 homeobox transcription factor is essential for gastrulation and early neural development. We generated Otx2 conditional knockout (cKO) mice to investigate its roles in telencephalon development after E9.0. We conducted transcriptional profiling and in situ hybridization to identify genes de-regulated in Otx2 cKO ventral forebrain. In parallel, we used ChIP-seq to identify enhancer elements, OTX2 binding motif, and which de-regulated genes are likely direct targets of Otx2 transcriptional regulation. We found that Otx2 was essential in septum specification; regulation of Fgf signaling in the rostral telencephalon; and medial ganglionic eminence (MGE) patterning, neurogenesis, and oligodendrogenesis. Within the MGE, Otx2 was required for ventral but not dorsal identity; this is the first demonstration of a transcription factor that contributes to regional patterning within the MGE. Microdissected subpallium (septum, MGE, and LGE ) from wildtype E12.5 CD-1 embryos was used in three independentanti-OTX2 ChIP-seq experiments.

2015-06-10 | E-GEOD-69724 | biostudies-arrayexpress

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