Project description:Human stem cell-derived hepatocyte-like cells (HLCs) offer an attractive platform to study liver disease and to test drugs. However, despite their advantages over other hepatocyte culture systems, HLCs lack several in vivo characteristics including cell polarity, which is critical for proper hepatocyte biology. We report a stem cell-based differentiation protocol that uses transwell filters to generate columnar polarized HLCs with clearly defined basolateral and apical membranes separated by tight junctions. Unlike conventional HLCs, polarized HLCs secrete cargo directionally, similar to hepatocytes in vivo. This novel system provides a powerful tool to study hepatocyte biology, disease mechanisms, genetic variation, and drug metabolism in a more physiologically relevant setting.
Project description:Transcriptional profiling of in vitro cultured mouse hepatocytes and hepatocyte-derived biliary cells demonstrated that in vitro cultured cells recapitulates the major transcriptional features of in vivo biliary cells and hepatocytes.
Project description:Whole genome expression of bone marrow deived hepatocytes after 1 and 5 months of transplantation are compared with that of primary hepatocytes and Lin- BM cells
Project description:During chronic liver injury, the ductular reaction generates hepatocytes. The aim of the analysis is to compare the phenotype of the DR-derived hepatocytes with that of native hepaotcytes in the same injured liver and control healthy hepatocytes.
Project description:Whole genome expression of bone marrow deived hepatocytes after 1 and 5 months of transplantation are compared with that of primary hepatocytes and Lin- BM cells Agilent one-color experiment,Organism:Mouse, Agilent Whole Genome Mouse 8x60k (AMADID: 028005 & 026986) , Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442)