Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Regulation of gene expression in undifferentiated spermatogonia by DDX5

ABSTRACT: Regulation of gene expression in undifferentiated spermatogonia by DDX5

PROVIDER: PRJNA524650 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Regulation of gene expression in undifferentiated spermatogonia by DDX5

Project description:The maintenance of spermatogenesis in adult males is dependent on a population of mitotic germ cells with self-renewal potential known as undifferentiated spermatogonia. Regulation of undifferentiated spermatogonia function is dependent on transcriptional and post-transcriptional mechanisms. We have identified an essential role for the RNA helicase DDX5 in undifferentiated spermatogonia through generation of a UBC-CreERT2;Ddx5flox/flox mouse model that allows tamoxifen-dependent Ddx5 ablation. To identify genes regulated by DDX5, we generated lines of cultured undifferentiated spermatogonia from these mice and treated cells with tamoxifen (TMX) to induce Ddx5 knockout or vehicle (VEH) as a control, and performed RNA-sequencing analysis to compare these conditions.

2019-04-02 | GSE127275 | GEO

PLZF and DDX5 interacting proteins in spermatogonia

Project description:DDX5, or PLZF co-immunoprecipitation in lysates from cultured undifferentiated spermatogonia followed by identification of eluted proteins using mass spectrometry. IgG control IPs included.

2019-02-24 | MSV000083476 | MassIVE

Identification of DDX5 target genes in spermatogonia

Project description:Sustained spermatogenesis in adult males and recovery of fertility following germ cell depletion are dependent on undifferentiated spermatogonia with self-renewal potential. Self-renewal of undifferentiated spermatogonia is dependent on a complex network of transcriptional and post-transcriptional regulatory factors. DDX5 is known to act as a transcriptional co-regulator in other systems through interactions with key transcription factors. Here, we performed ChIP-Seq to identify target genes of DDX5 in cultured undifferentiated spermatogonia in order to elucidate its role in maintenance of the male germline.

2019-04-02 | GSE110347 | GEO

Gene expression in undifferentiated spermatogonia

Project description:Gene expression in undifferentiated spermatogonia

| PRJNA605339 | ENA

Gene expression in undifferentiated spermatogonia

Project description:RNA-seq of sorted undifferentiated spermatogonia from synchronized postnatal testes.

2020-07-22 | GSE144923 | GEO

DAZL targets in undifferentiated spermatogonia

Project description:DAZL targets in undifferentiated spermatogonia

| PRJNA605338 | ENA

Translational profiling in undifferentiated spermatogonia

Project description:Translational profiling in undifferentiated spermatogonia