Project description:We used RNA sequencing to identify differentially expressed genes in whole mouse placentas. Pregnant mice were fed either a 20% or 6% protein (isocaloric) two-weeks before mating and throughout pregnancy. Placental lysates are derived from C57BL/6 conceptuses at E16.5 of gestation.
Project description:Total RNA isolated from maternal serum (drawn between 36-37 weeks gestation)from pregnancies with a growth restricted infant defined as an Individualised birthweight centile (IBC) < 3rd matched to a control pregnancy (IBC 20-80th), n=4 per group with 2 female and 2 male infants per group. Maternal serum miRNAs profiled using miRCURY LNA Universal RT miRNA PCR Human panel I + II to determine biomarker potential for prediction of growth restriction and sexually dimorphic patterns.
Project description:Placenta accreta is a major cause of maternal morbidity and mortality in modern obstetrics. The study aims to identify the differently expressed lncRNA and mRNA in placenta accreta patients compared with controls and to determine biological pathways, which provides a novel insight ingto thepathogenesis of placneta accreta.
Project description:Placenta accreta is a major cause of maternal morbidity and mortality in modern obstetrics. The study aims to identify the differently expressed proteins in placenta accreta patients compared with controls and to determine biological pathways, which provides a novel insight into the pathogenesis of placneta accreta.
Project description:The placenta has a critical role in fetal growth, with many key functions regulated by genomic imprinting. With the recent description of polymorphic placenta-specific imprinting, the molecular mechanisms leading to this curious epigenetic phenomenon are unknown, as is their involvement in pregnancies complications. Profiling ubiquitous and placenta-specific imprinted differentially methylated regions (DMRs) exposed isolated aberrant methylation at ubiquitous DMRs as well as abundant hypomethylation at placenta-specific DMRs. Analysis of underlying chromatin at polymorphic placenta-specific imprinted DMRs revealed biallelic enrichment of histone H3K4 methylation, a modification normally mutually exclusive with DNA methylation. Furthermore, characterisation of expression in intrauterine growth restricted samples (IUGRs) uncovered coordinated deregulation of the GPR1AS1-ZDBF2-ADAM23 locus. Our results emphasize that methylation is less stable at placenta-specific imprints compared to their ubiquitous counterparts and that further work is required to determine if these differences are the IUGR cause or reflect unique adaption of the placenta epigenome to developmental stresses.
Project description:We provide the tissue-level human placental transcriptomes from two term uncomplicated pregnancies. Tissue was collected at term C-section (no labor), from villous part of the placenta.
Project description:The purpose of this study was to determine the difference of the miRNA profiles between normal and preeclamptic placenta. Ten placental samples were analyzed. Six were from preeclamptic patients and four were from normal pregnancies.
Project description:Trophoblast organoids (TOs) hold great promise for elucidating human placental development and function. By deriving TOs in on-going pregnancies using chorionic villus sampling (CVS), we established a platform to study trophoblast differentiation and function in early pregnancy, including pregnancies with different fetal genetic abnormalities. We addressed cellular heterogeneity of CVS-derived TOs by providing a single-cell transcriptomic atlas and show that CVS-TOs recapitulate key aspects of the human placenta, including syncytial fusion and hormone synthesis. This study demonstrates the utility of trophoblast organoids for investigating genetic defects in the placenta and describes an experimental platform for future personalized placental medicine approaches, including genotype-phenotype mapping.