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Rhodobacter capsulatus SB1003 under singlet oxygen stress

ABSTRACT: Rhodobacter capsulatus SB1003 under singlet oxygen stress

PROVIDER: PRJNA554301 | ENA |

REPOSITORIES: ENA

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Rhodobacter capsulatus SB1003 under singlet oxygen stress

Project description:Global transcriptome analyses at growth before and after 10 min of photooxidative stress were applied to monitor stress dependent gene expression in the alpha-proteobacterium Rhodobacter capsulatus. Transcriptome profiles of pigmented cultures with high aeration were monitored before and after the onset of singlet oxygen stress.

2020-01-24 | GSE134200 | GEO

Rhodobacter sphaeroides WT 2.4.1 under singlet oxygen stress

Project description:Transcriptional and translational profiling of R. sphaeroides WT 2.4.1 under singlet oxygen stress compared to control (no stress) conditions

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Rhodobacter sphaeroides WT 2.4.1 under singlet oxygen stress

Project description:Transcriptional and translational profiling of R. sphaeroides WT 2.4.1 under singlet oxygen stress compared to control (no stress) conditions RNA samples collected at time-point zero and at different time-points after singlet oxygen stress were analyzed by two-color microarrays

2012-11-14 | E-GEOD-42244 | biostudies-arrayexpress

Rhodobacter sphaeroides 2.4.1 pPos19 versus pBBR under singlet oxygen stress

Project description:To learn more about the function of Pos19, we constructed an over-expression strain. For this purpose, the Pos19 gene together with its native RpoE promoter was cloned into the middle-copy plasmid pBBR1 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a singlet oxygen-induced Pos19 over-expression compared to an empty vector control. Total RNA from the over-expression (pPos19) and from the empty vector control (pBBR) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate Pos19-induced changes on transcriptome level.

2017-02-09 | GSE81718 | GEO

Rhodobacter sphaeroides 2.4.1 pSurS versus pBBR under singlet oxygen stress.

Project description:To learn more about the function of SurS, we constructed an over-expression strain. For this purpose, the surS gene together with its native RpoE promoter was cloned into the middle-copy plasmid pBBR1 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a singlet oxygen-induced SurS over-expression compared to an empty vector control. Total RNA from the over-expression (pSurS) and from the empty vector control (pBBR) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate SurS-induced changes on transcriptome level. RNA samples collected after 7 min of singlet oxygen stress were analyzed by two-color microarrays. Each microarray contains a pool of three independent biological replicates for each sample.

2014-07-04 | E-GEOD-59046 | biostudies-arrayexpress

Rhodobacter sphaeroides 2.4.1 pPos19 versus pBBR under singlet oxygen stress.

Project description:Rhodobacter sphaeroides 2.4.1 pPos19 versus pBBR under singlet oxygen stress.

| PRJNA254205 | ENA

Rhodobacter sphaeroides 2.4.1 pRKSorX144 versus pRK415 under singlet oxygen stress.

Project description:To learn more about the function of SorX, we constructed an over-expression strain. For this purpose, the sorX gene together with its 28-bp upstream region was cloned into the over-expression vector pRK4352 and expressed in the R. sphaeroides wild-type 2.4.1 background. The corresponding strain consequently exhibits a SorX over-expression that is driven by the constitutive 16S rRNA (RSP_4352) promoter. The over-expression strain was compared to an empty vector control (pRK415). Total RNA from the over-expression (pRKSorX144) and from the empty vector control (pRK415) strain was extracted after 7 min of singlet oxygen stress and used for microarray analysis to investigate SorX-induced changes on transcriptome level.

2017-01-01 | GSE79421 | GEO

R. sphaeroides pBBBRUpsMx2 vs. R. sphaeroides pBBR under singlet oxygen stress

Project description:Transcriptional profiling of R. sphaeroides pBBRUpsMx2 compared to control R. sphaeroides pBBR under high oxygen and singlet oxygen conditions.

2017-01-01 | GSE87789 | GEO

Transcriptome and proteome analyses of the CtrA regulon and growth-phase regulation in Rhodobacter capsulatus

Project description:The samples in this series were used to analyze the transcriptome of the CtrA regulon using wild type (SB1003) and ctrA mutant (SBRM1) strains of Rhodobacter capsulatus. As well, the transcriptome of growth phase regulation in R. capsulatus SB1003 between log and stationary phases was determined.

2010-04-08 | GSE18149 | GEO

RpoE regulon in Rhodobacter sphaeroides

Project description:In Rhodobacter sphaeroides a transcriptional response to the reactive oxygen species singlet oxygen is controlled by the group IV sigma factor RpoE and the anti-sigma factor ChrR. In this study, we integrated various large datasets to identify genes within the singlet oxygen stress response that contain RpoE-dependent promoters within R. sphaeroides. Transcript pattern clustering and a RpoE-binding sequence model were used to predict candidate promoters that respond to singlet oxygen stress in R. sphaeroides. These candidate promoters were experimentally validated to nine R. sphaeroides RpoE-dependent promoters that control the transcription of 15 genes activated by singlet oxygen.

2010-07-01 | GSE22576 | GEO

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