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Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection

ABSTRACT: Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection

PROVIDER: PRJNA565803 | ENA |

REPOSITORIES: ENA

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Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection

Project description:Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection

| PRJNA565802 | ENA

Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection

Project description:The full neutrophil heterogeneity and differentiation landscape remains incompletely characterized. Here we profiled more than 25,000 mouse differentiating and mature neutrophils using single-cell RNA sequencing to provide a comprehensive transcriptional landscape of neutrophil maturation, function, and fate decision in their steady state and during bacterial infection. Eight neutrophil populations (including the GMP population) were defined by distinct molecular signatures, including a new circulating mature neutrophil population highly expressing interferon-stimulated genes. The three mature peripheral blood neutrophil subsets arise from distinct maturing bone marrow neutrophil subsets, a novel mechanism that highlights the complex and precise regulation of neutrophil production. Neutrophil heterogeneity and differentiation are driven by both known and uncharacterized transcription factors. Neutrophils gradually acquire microbicidal capability as cells traverse the transcriptional landscape, representing an evolved mechanism for fine-tuned regulation of an effective but balanced neutrophil response. Bacterial infection reprograms the genetic architecture of neutrophil population, alters dynamic transition between each subpopulation, and primes neutrophils for augmented functionality without affecting overall heterogeneity. Bacterial infection-induced emergency granulopoiesis is mediated by augmented proliferation of early-stage neutrophil progenitors and accelerated post-mitotic maturation. In summary, single-cell transcriptomics enabled the reconstruction of neutrophil differentiation and maturation trajectories and uncovered neutrophil subpopulations, gene pathways, and regulators of neutrophil function and fate decisions. These data establish a reference model and general framework for studying neutrophil-related disease mechanisms, biomarkers, and therapeutic targets at single-cell resolution.

2020-05-27 | GSE137539 | GEO

Tissue neutrophil heterogeneity

Project description:Tissue neutrophil heterogeneity

| PRJNA594618 | ENA

Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection.

Project description: Not available

| S-EPMC7442692 | biostudies-literature

Functional evaluation of neutrophil of patients at high risk for infection treated with monocyte/ neutrophil apheresis

Project description:Interventions: monocyte/neutrophil apheresis non monocyte/neutrophil apheresis Primary outcome(s): Evaluation neutrophil function of patients at high risk for postoperative infection treated with monocyte/neutrophil apheresis Study Design: Parallel Non-randomized

| 2627605 | ecrin-mdr-crc

H2R signaling maintains neutrophil homeostasis

Project description:H2R signaling maintains neutrophil homeostasis

| PRJNA683590 | ENA

Functional evaluation of neutrophil of patients at high risk for infection treated with multiple monocyte/ neutrophil aphaereses

Project description:Interventions: multiple monocyte/neutrophil apheresis non monocyte/neutrophil apheresis Primary outcome(s): Evaluation neutrophil function of patients at high risk for postoperative infection treated with monocyte/neutrophil apheresis Study Design: Parallel Non-randomized

| 2628620 | ecrin-mdr-crc

Single-cell transcriptome profiling reveals neutrophil heterogeneity in homeostasis and infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-05-26 | GSE137540 | GEO

Tissue neutrophil heterogeneity in germ free mice

Project description:Tissue neutrophil heterogeneity in germ free mice

| PRJNA597017 | ENA

Semi-quantitative proteomics enables mapping of global neutrophil dynamics following influenza virus infection

Project description:Lung neutrophils are causally associated with IAV-induced disease severity. Less is known about the repertoire of lethal IAV-associated neutrophil proteins or about how global changes in different neutrophil compartments are coordinated following lethal IAV infection. Here, we use semi-quantitative proteomics to characterize dynamic alterations in BM, blood and lung neutrophils at homeostasis or following a lethal IAV infection, with a secondary aim of identifying lung neutrophil-derived proteins which are selectively induced following IAV infection. Our findings identify bone marrow neutrophil maturation as the key site of anti-viral activity induction, with further upregulation or release of both anti-viral and antimicrobial effectors occurring following lung tissue infiltration.

2019-05-29 | PXD012681 | Pride

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