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Single cell sequencing of mice brain after stereotactic injection treatment

ABSTRACT: Single cell sequencing of mice brain after stereotactic injection treatment

PROVIDER: PRJNA565984 | ENA |

REPOSITORIES: ENA

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Single cell sequencing of mice brain after stereotactic injection treatment

Project description:We report the application of single cell sequencing technology for high-throughput profiling in mice brain cells.In order to search for SCZ-related molecular pathways in different brain cell types affected by immune activation, we first activated the peripheral immune system by intraperitoneal injection (IPI) of lipopolysaccharide (LPS) into adolescent mice twice on post-natal day 45 and 46, and collected the serum from the mice on the third day. Then we performed intracerebroventricular injection (ICVI) of 15 ul of serum from the immune-activated mice. After ICVI of immune-activated serum for one week, we carried out single-cell transcriptome sequencing on the brains from the mice. This study provides characterization of diverse cell populations towards different treatments.

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Project description:The aim of the experiment was to identify differences in gene expression in the whole brain hemisphere due to the presence of a brain tumor. At 21 days after a stereotactic injection of 5 x 10E4 rat C6 glioma cells into the right striatum of the rat the animals were sacrificed and the tumor-bearing brain hemispheres were isolated. The control animals were left untreated. The focus of the experiment was on the changes in gene expression due to the immune cells infiltrating the tumor and the surrounding brain parenchyma.

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