Ontology highlight
ABSTRACT:
PROVIDER: PRJNA597200 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Nannochloropsis gaditana is a microalgae of the phylum eustigmatophyceae that has been reported from fresh and brackish waters. This species has been widely cultured, mainly directed to biofuel production, due to its capacity to produce and accumulates high amounts of lipids under different culturing conditions. Furthermore, nowadays N. gaditana is being used as fish and mollusc food in aquaculture facilities. Besides, microalgae are recognized protein producers, thus, are posited as an alternative protein source that could be very valuable in areas such as agri-food or biomedicine. In this sense, seas and oceans had showed their great potential for innovation, being the main focus of the initiative Blue Growth from EU. Thereby, marine biotechnology will provide of new pharmaceuticals or industrial products from marine biomass. In order to study the whole proteome of Nannochloropsis gaditana, a proteomic approach was initiated using fresh and atomized microalgae samples, as the main commercial forms. Around 7500 peptides were detected, getting 1950 protein identification hits. Qualitative and quantitative differences were analysed. The identified proteins were categorized according to gene ontology classification. In this study, it has been developed and described the first proteomic analysis of the microalgae Nannochloropsis gaditana, containing an important number of identified proteins that may have a relevant role in different agri-food and biomedical processes.
2018-11-07 | PXD008499 | Pride
Project description:Epigenetic modifications have not been well characterized in most microalgae species, particularly those used for biofuel production. We report the first analysis of DNA methylation of the species Picocholorum soloecismus across its growth cycle using whole-genome bisulfite sequencing. DNA methylation occurs in all three cytosine contexts (CpG, CHH, CHG) in P. soloecismus. While global DNA methylation is low overall (~1.15% of the 15 MB genome), methylation occurs appreciable quantities (12.1%) in CpG dinucleotides in a bimodal distribution. The P. soloecismus genome becomes hypomethylated during the growth cycle (across several days in culture). 5-aza-2’-deoxycytidine (5AZA) treatment was used to alter DNA methylation patterns, which resulted in altered algal physiology and site specific changes in CpG DNA methylation.
2020-08-01 | GSE155500 | GEO
Project description:To investigate the mechanism by which the microalgae-yeast co-culture system promotes wastewater denitrification. We concluded that microalgae and yeast exhibit a mutually beneficial relationship in the co-culture system. Microalgae nitrogen metabolism can be influenced by both miRNA and mRNA, and the presence of yeast stimulates gene expression in microalgae.
2023-07-12 | GSE231399 | GEO
Project description:We conducted a culture experiment by deeply submerging plants in swine wastewater in culturing Iris tectorum and co-culturing Iris tectorum and Dictyosphaerium sp., and found that the plants grew sub-normal in the plant-microalgae co-culture while the plants were dead after 21 days in the plant culture. We generated a comprehensive RNA-seq dataset from the submerged Iris tectorum leaves in both the plant culture and the plant-microalgae co-culture, aiming at providing information on the response mechanisms of the plants to waterlogging stress. Besides raw reads of the RNA-seq dataset, we used DEseq2 algorithms to detect the differently expressed genes in the plants between the different cultures. Additionally, we performed the plant disease resistance gene analysis for all the differentially expressed genes.
2018-11-03 | GSE122095 | GEO
Project description:Harmful algal blooms are induced largely by nutrient enrichment common in warm waters. An increasingly frequent phenomenon is the “red tide”: blooms of dinoflagellate microalgae that accumulate toxins lethal to other organisms in high doses. Here, we present the de novo assembled genome (~4.75 Gbp) of Prorocentrum cordatum, a globally abundant, bloom-forming dinoflagellate, and the associated transcriptome, proteome, and metabolome data from axenic cultures to elucidate the microalgal molecular responses to heat stress. We discovered, in a high-G+C genome with long introns and extensive genetic duplication, a complementary mechanism between RNA editing and exon usage that regulates dynamic expression and functional diversity of genes and proteins, and metabolic profiles that reflect reduced capacities in photosynthesis, central metabolism, and protein synthesis. These results based on multi-omics evidence demonstrate the genomic hallmark of a bloom-forming dinoflagellate, and how the complex gene structures combined with multi-level transcriptional regulation underpin concerted heat-stress responses.
2023-10-18 | PXD046193 | Pride