Proteomics,Multiomics

Dataset Information

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Proteomics characterization of the non-model microorganism: Nannochloropsis gaditana.


ABSTRACT: Nannochloropsis gaditana is a microalgae of the phylum eustigmatophyceae that has been reported from fresh and brackish waters. This species has been widely cultured, mainly directed to biofuel production, due to its capacity to produce and accumulates high amounts of lipids under different culturing conditions. Furthermore, nowadays N. gaditana is being used as fish and mollusc food in aquaculture facilities. Besides, microalgae are recognized protein producers, thus, are posited as an alternative protein source that could be very valuable in areas such as agri-food or biomedicine. In this sense, seas and oceans had showed their great potential for innovation, being the main focus of the initiative Blue Growth from EU. Thereby, marine biotechnology will provide of new pharmaceuticals or industrial products from marine biomass. In order to study the whole proteome of Nannochloropsis gaditana, a proteomic approach was initiated using fresh and atomized microalgae samples, as the main commercial forms. Around 7500 peptides were detected, getting 1950 protein identification hits. Qualitative and quantitative differences were analysed. The identified proteins were categorized according to gene ontology classification. In this study, it has been developed and described the first proteomic analysis of the microalgae Nannochloropsis gaditana, containing an important number of identified proteins that may have a relevant role in different agri-food and biomedical processes.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Nannochloropsis Gaditana

SUBMITTER: Francisco Fernández-Acero  

LAB HEAD: Francisco Javier Fernández Acero

PROVIDER: PXD008499 | Pride | 2018-11-07

REPOSITORIES: Pride

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Publications

Valorisation of the microalgae Nannochloropsis gaditana biomass by proteomic approach in the context of circular economy.

Fernández-Acero Francisco Javier FJ   Amil-Ruiz Francisco F   Durán-Peña María Jesús MJ   Carrasco Rafael R   Fajardo Carlos C   Guarnizo Palmira P   Fuentes-Almagro Carlos C   Vallejo Roberto A RA  

Journal of proteomics 20181029


Nannochloropsis gaditana is a non-flagellated microalgae that has been widely used for different purposes, mostly related with the industrial production of biofuels or aquiculture. However, in order to increase the economic viability of the obtained microalgae biomass from a production plant coupled to a coal power plant, a proteomic approach was initiated by using fresh and atomized microalgae samples, as the main used commercial forms. Above 51,000 high quality spectra were obtained per sample  ...[more]

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